For the last four years you have worked for a large drug manufacturing company as a drug development research scientist. Recently, you were appointed as lead scientist for one of the company’s research teams and asked to evaluate a group of soil Actinobacteria as a potential source of antibiotics against the pathogenic bacterium Streptococcus pneumoniae. This bacterium causes pneumonia in humans. The development team has already extracted two promising drugs (R16 and R22). In order to test the effectiveness of these two potential antibiotics, you call you team together and devise an experiment, using laboratory mice. In preparation for the experiment your technicians have just injected the mice (200 total individuals – divided equally between the two treatment drugs) with the Streptococcus pneumoniae bacterium. Two weeks into the experiment, as you evaluate the preliminary data, you notice that 85% of the infected mice treated with 20 mg, of R22, 3 times a day, for 10 days have recovered fully from the bacterial infection; however, 10% of the mice treated with R22 have developed a chronic liver condition. The same treatment regime using R16 yields similar results (83% recovery but 12% of the mice have developed a mild, treatable rash on their forelimbs).
1.Which of the following would be the independent variable in your experiment?
a.the total number of mice used in the experiment
b.the concentration of Streptococcus pneumoniae cells injected in the experimental group of mice.
c.the two drugs (R16 and R22).
d.the amount of water available to each mouse during a 24 hour period
e.none of the above are correct
2.Of the following possible dependent variables which one most specifically applies to your experiment?
a.the temperature in the “mouse house” where you are conducting your experiment.
b.changes in blood pH between the experimental and control groups.
c.percent survival of mice in the experimental and control groups.
d.differences in urination frequency between the experimental and control groups.
e.none of the above are correct
3.Which of the following factors must be held constant between the control and experimental groups in your experiment?
a.the concentration of Streptococcus pneumoniae injected into the mice
b.the core body temperature of the mice
c.the amount of R16 and R22 administered
d.the urination frequency of the mice
Which of the following would be a valid hypothesis for your experiment?
a.Mice commonly die when infected with Streptococcus pneumoniae.
b.Actinobacteria are common soil microorganisms.
c.The drugs R16 and R22 are not effective antibiotics against Streptococcus pneumoniae.
d.Lung tissue in mice is not susceptible to Streptococcus pneumoniae.
5.Based on the preliminary data from your experiment with mice, which drug do you think holds the greatest promise for treating pneumonia in humans?
a.R16
b.R22
c.A combination of R16 and R22
d.Neither
2006-09-12
16:19:59
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7 answers
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asked by
Anonymous