hello friends can u tell me which the idea medium for culturing drosophila along with the procedure ?

Question

drosophila fruit fly culture

Answer 1

I worked in a research lab where I made fly food a few years ago, and here is a recipe similar to what we used. Instead of molasses though, we used glucose and sucrose. But I think this would work as well. The chemicals are inhibitors to make sure bacteria/mold doesn't grow on the media. You will need a big pot to make it in, and then you will need to put it into either bottles or vials or both, depending on how you are planning on keeping the flies. If you want to use vials - there are contraptions that you can buy to pour a whole tray of vials at once. I have the website below, and I might also recommend looking through papers in drosophila and maybe contacting the professors to see how they make their fly food. Anyways - here is the website:

Answer 2

c. mcd. is right about the method and recipe but there are also other recipes, you can find them here (this is THE resource on fly food recipes:)

http://flystocks.bio.indiana.edu/Fly_Work/media-recipes/media-recipes.htm

here is also another great recipe, which I have found to result in very quick growth of drosophila:

1220 ml water
12 g agar
100 g brewer's yeast
100 g glucose

propionic acid/phosphoric acid mixture
moldex (10% solution in ethanol)

mix agar & water and bring to a boil. add glucose and brewer's yeast and beat in with a whisk, and turn down heat. simmer for 20 minutes. add 10 ml of acid mix and 10 ml of moldex, mix for another minute. cool to 42C before pouring. whisk before pouring to prevent separation.

you can also buy instant drosophila medium here:
http://www.sargentwelch.com/product.asp_Q_pn_E_WL54421_EA_A_Instant+Drosophila+Medium_E_