All categories - 31 March 2007

always wondered

2007-03-31 02:24:38 · 13 answers · asked by blaine g 1 in Mathematics

2007-03-31 02:24:24 · 7 answers · asked by miriam r 1 in Singles & Dating

When I run an SDS-PAGE and western blot and develop the films, I get these dark staining bands on the molecular weight markers. But they aren't necessarily all clear "bands", they are more like dark areas in the lane where the gel ran and then a couple specific bands. Is it a problem at the gel stage, the transfer, blocking, incubating with antibodies.....what could it be? So this happened and I stripped the original blots and reblotted them, and I got the same results.
Why are the antibodies are sticking to the MWM?!?!?!

2007-03-31 02:23:54 · 1 answers · asked by Risa M 1 in Biology

what kind of wine would go with it? Merlot?

2007-03-31 02:23:07 · 10 answers · asked by Dave 6 in Beer, Wine & Spirits

I think its more like China....and you ?

2007-03-31 02:22:59 · 9 answers · asked by Anonymous in Environment

2007-03-31 02:22:47 · 14 answers · asked by Cheng 2 in Lesbian, Gay, Bisexual, and Transgender

stay silent now?

2007-03-31 02:22:46 · 7 answers · asked by JoeFriday 1 in Current Events

and its next to impossible to avoid this situation... also .... you feel you are thru with him but seeing him everyday just presses the wrong button . he is married so are you ... but .... if /when he gets flirty with his wife .. you feel awkward ... oh boy can someone tell me a way to not get affected by this person????

2007-03-31 02:22:45 · 7 answers · asked by barley 2 in Singles & Dating

help me get to level four?

I promise I'll be good if he does!

2007-03-31 02:22:37 · 16 answers · asked by Anonymous in Religion & Spirituality

2007-03-31 02:22:35 · 1 answers · asked by zfdh r 1 in Books & Authors

i get terribable (i cnt spell lol) heart burn and i wanted to know some heartburn remidies that you can find in the house like types of food or something. I cant afford all this fancy tablets and stuff so lil help?

2007-03-31 02:22:12 · 9 answers · asked by imortal_emo 2 in Other - Health

The horn on my 18 year old Volvo just started beeping by it self. I just had the brakes fixed and don't want
to take it back to the shop as that was costly enough. Its a 240 wagon. Also had the back wiper fixed too as it had stopped working. Can I fix the horn myself with limited mechanical knowledge?

2007-03-31 02:22:04 · 5 answers · asked by greenfrogs 7 in Volvo

i really liked the profound message behind this movie.. which character do u sympathize the most? and why?

2007-03-31 02:21:24 · 12 answers · asked by [MiA] 2 in Polls & Surveys

When do u know that you are obsessed over a girl? I have a few pictures, have her phone number (through an old school driectory) and i have dreamt about her.....is that obsessed? My parents think i am. Just so you know....i havent seen her in 4 years, and we live in two different countries....

2007-03-31 02:21:04 · 8 answers · asked by the ghoul 2 in Friends

I just found an article from "Creation" magazine which spells out plainly that Darwin did NOT recant and embrace Christianity on his death-bed. It was written by one Russell M. Grigg, a Creationist Chemist (!) and Missionary, in 1995.

I know, outrageous! If anyone wants me I'll be recovering in the corner...

http://www.answersingenesis.org/creation/v18/i1/darwin_recant.asp

2007-03-31 02:20:55 · 10 answers · asked by Anonymous in Religion & Spirituality

I want to be bright like a programmer, Nietzsche.
But I don't want to be different, extinct from people around me. Please convince me to meditate.

2007-03-31 02:20:51 · 8 answers · asked by Anonymous in Religion & Spirituality

I want to make a short list of those Open Source or free apps which make a developer's live very much easier. Not a massive exhaustive list (for my site - http://www.nerds-central.com, and not things like script engines and compilers. Just the 'must have grab bag'. It is Windows a centric. Here is where I have gotten so far:

msys
ultra vnc
notepad++
gimp
Process Explorer (procexp)
firefox
gvim
jmeter
skype
putty
filezilla
inkscape
wincvs
windump
netcat

Once I have the list all worked out, I'll crate links to where to get them, a quick review and 'why use it' section.

Why - because I always trying to support people on the phone or Skype and need to have a page where I can say - get these then call back and I can help properly :)

Any suggestions welcome - for example - is XMLPad any good?

Thanks - AJ
http://www.nerds-central.com

2007-03-31 02:20:44 · 1 answers · asked by Alex Turner 2 in Programming & Design

wake me up so early on a saturday morning?

2007-03-31 02:20:40 · 11 answers · asked by Anonymous in Religion & Spirituality

and if so, how can you "love" someone you've never met in the flesh?

isn't it just meaningless to profess otherwise? and if that's true, what do you really get out of God's love?

2007-03-31 02:20:19 · 10 answers · asked by Brendan G 4 in Religion & Spirituality

2007-03-31 02:20:07 · 5 answers · asked by Me 2 in Diet & Fitness

My 6 month old son doesn't seem to have any teeth coming through,although he is dribbling like mad ,chewing on everything,and has had a bit of a cold and fever for a while.
He doesn't seem to be in any pain but has become very restless at night and not sleeping for more than about 2 hours at a time.Could all this be connected.

2007-03-31 02:19:58 · 9 answers · asked by Anonymous in Newborn & Baby

Six months ago after having a heart attack, an individual was taken to the cath lab, angioplasty was performed, and three stents were deployed. The patient has done well. He, however, is now concerned about the risk of late complications and wants compensation, saying "If the doctors increased my chances for having another heart attack, even so slightly, I feel it's fair to ask for some kind of compensation."

Do you agree, is this reasonable? Do you have similiar expectations?

See link for the entire context: http://answers.yahoo.com/question/index?qid=20070329023928AADJdHO&r=w&show_comments=true&pa=FZB6NWHjDG3N56z6v_2zWewIa4bNThbNoQ3WDDwg8pp8OiTTgOC2og--&paid=add_comment#openions

2007-03-31 02:19:57 · 8 answers · asked by c_schumacker 6 in Other - Diseases

I want to be able to view my frame content but not the frame it is in.

2007-03-31 02:19:38 · 4 answers · asked by moonguardianluna 3 in Programming & Design

2007-03-31 02:19:34 · 41 answers · asked by latymer_crown 6 in Polls & Surveys

2007-03-31 02:19:26 · 3 answers · asked by satti 2 in Cell Phones & Plans

a) 0.100 M HONH3Cl

b) A mixture containing 0.100 M HONH2 and 0.100 M HONH3Cl

Please help me in solving this problem. Thanx in advance.....

2007-03-31 02:19:13 · 3 answers · asked by Anonymous in Chemistry

2007-03-31 02:19:12 · 24 answers · asked by Anonymous in Polls & Surveys

2007-03-31 02:18:38 · 4 answers · asked by oobb123 1 in Credit

what is greek gyro (Lamb,Chicken....)..Is the Sandwich..Salad..Meal???
Any pictures..

2007-03-31 02:18:17 · 8 answers · asked by jill 3 in Cooking & Recipes

her name is Jessica H she isn't answering a question but asking you to join a motorcycle club

this is the link she was showing people

http://hometown.aol.com/coolbiker0165/in...

2007-03-31 02:18:13 · 5 answers · asked by Whitt 5 in Security