anyone familiar with XTT or done XTT assay before? according to product manual, the more viable cells, the more XTT rings it cleaves and more orange formazan accumulate. therefore when the OD is read, absorbance is higher for samples with more live cells, right? absorbance is lower for the wells with more dead cells right? but my result shows the other way round. the more pathogen protein i put in, the higher the absorbance.
I used a medium with phenol red (although not recommended). i suspect my protein change the pH of medium, thus it change colour. but i also have control wells that contain cells, medium and protein (without phenol red), the protein doesn't chnage the colour(pH) of medium. the readings for the wells (no XTT) is consistant.
Is the graph i got correct (absorbance increase when pathogen protein concentration is increased)?
2006-11-02
19:16:13
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1 answers
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asked by
snow_cat
1
in
Biology