In digesting a certain plasmid with restriction endonucleases, how do we explain if
1. One (biggest) fragment from the digestion has a size larger than the plasmid itself, and this fragment is outside the range of the molecular markers used for the digestion..contamination?
2. Why a lane for the uncut plasmid has more than one band?(since no enzyme is added, no digestion should have occurred and only one band is expected...)
it would be great if you can provide me the source of info..thanks~
2007-01-21
05:24:40
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2 answers
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asked by
KeenaUsas
1