I'm working on a biology lab, trying to analyse the data, etc., and having trouble coming up with the slope of the results. The graph is the concentration of ortho-quinone as a measure of light absorbance (using a photospec) over time. The lab dealt with tyrosinase reacting with a catechol substrate, so ortho-Q was the product. Basically we were just measuring the rate of the reaction, which is the slope of the line (we tested the sample in the spec every 30s for 10 mins). MY PROBLEM IS...I'm unsure of the units to use to get the slope. If I use delta-X in seconds (600) and delta-y in percentage (about 40%), I get a different slope than if I use minutes and decimals between 0 and 1. What should I do? I'm just a bio student, not a scientist or anything, and I haven't been in school in years so my math certainly needs work, I guess...Any help is appreciated, and if you need to see the actual graph and data table (Excel file), let me know and I'll email. Thanks!
2007-02-08
06:02:14
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2 answers
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asked by
Chef Dom
1