how does chromatography work?

Question

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Answer 1

I think of chromatography more as a "sieve" effect. The colors of ink/pigment are initially all mixed together when you put them on the paper, but each color has differences in characteristics, such as the molecule sizes and molecular weight. When a solution (water, acetone) is put onto the paper it's pulled through the paper fibers and the pigments along with it. The larger/heavier pigments get caught up by the fibers and can't move as far or as quickly as the smaller, lighter ones - sort of like materials dissolved in water passing through a sieve or strainer, but larger particles are held back.

Answer 2

An analogy which is sometimes useful is to suppose a mixture of bees and wasps passing over a flower bed. The bees would be more attracted to the flowers than the wasps, and would become separated from them. If one were to observe at a point past the flower bed, the wasps would pass first, followed by the bees. In this analogy, the bees and wasps represent the analytes to be separated, the flowers represent the stationary phase, and the mobile phase could be thought of as the air. The key to the separation is the differing affinities among analyte, stationary phase, and mobile phase. The observer could represent the detector used in some forms of analytical chromatography. A key point is that the detector need not be capable of discriminating between the analytes, since they have become separated before passing the detector.

Answer 3

skinny Layer Chromatography (TLC) is comparable to Paper different than fairly than utilising a stationery part of paper it makes use of a skinny layer of adsorbent (silica gel or cellulose) on a flat, unreactive substrate. that's consequently greater constructive because it runs swifter and has clearer separations