Does anyone know where I could download a good Gel Electrophoresis/SDS-PAGE animation.?

Question

Especially for protein separation and I want to know the general porcedure.

Answer 1

Try this one:
http://bcs.whfreeman.com/lodish5e/pages/bcs-main.asp?v=category&i=03010.04&o=|01000|03000|&ns=0

Answer 2

http://www.bme.gatech.edu/vcl/SDS_PAGE/Background/runninggel.htm

there is a great animation of a running sds polyacrylamide gel on here

Gel electrophoresis is a group of techniques used to separate molecules based on physical characteristics such as size, shape, or isoelectric point.

Gel electrophoresis of large DNA or RNA is usually done by agarose gel electrophoresis
prepare gel of usually 1%, smaller molecules may require larger such as 2%. Add 1 µl ethidium bromide (mutagen) stock per 10 ml gel solution, and stir.
pour into gel rack and add comb to provide wells.When the gel has cooled down and become solid, carefully remove the comb. the gel is then placed in 0.5x TBE so it is fully covered and the cathode(-) is near the wells with the anode(+) at the bottom
After the gel has been prepared, inject about 25 µl of stained DNA (a DNA ladder is also highly recommended). Apply current of typically 100 V for 30 minutes with 15 ml of gel. When the ladder dye approaches the end of the gel, the current is stopped. It is now possible to visualize the DNA (stained with ethidium bromide) with ultraviolet light.

sodium dodecyl sulfate polyacrylamide gel is used for proteins - The solution of proteins to be analyzed is first mixed with SDS, an anionic detergent which denatures secondary and non–disulfide–linked tertiary structures, and applies a negative charge to each protein in proportion to its mass. It linearizes the proteins so that they may be separated strictly by molecular weight (primary structure, or number (and size) of amino acids). binding ratios mean the samples have an approximately uniform mass:charge ratio for most proteins, so that the distance of migration through the gel can be assumed to be directly related to only the size of the protein.

there is a surpisingly good bit on wikipedia - http://en.wikipedia.org/wiki/SDS-PAGE

hope this helps :) :) :) :)

Answer 3

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