what is the recommended polyacrylamide gel percentage for protein size 15kD and 34kD?
do any one have a website to recommened?
previously i tried using 7.5% gel, is that really recommended or am i to use higher percentage?
thank you in advanced. : )
2007-12-21 03:03:49 · 4 answers · asked by Anonymous in Science & Mathematics ➔ Biology
You want to run a MORE dense gel, not a less dense one.
I look at p15 (15kDa) on a 4-20% gradient pre-cast gel, purchased from the company listed above.
You want to have 15-34 end up in the CENTER of the gel. on a 7.5%, you're looking at it running right off the bottom.
2007-12-21 04:14:26 · answer #1 · answered by LabGrrl 7 · 0⤊ 0⤋
Gel Percentage Protein Size
2016-12-14 05:16:48 · answer #2 · answered by Anonymous · 0⤊ 0⤋
I would use a higher percentage, perhaps 12-15%. The Bio-Rad website below has a nice graphic depicting the approximate migration of different sized proteins on gels of different acrylamide percentages. Try that link.
http://biorad.com/B2B/BioRad/product/br_category.jsp?BV_SessionID=@@@@1302544854.1198253768@@@@&BV_EngineID=cccfaddmlffhfkjcfngcfkmdhkkdfll.0&categoryPath=%2fCatalogs%2fLife+Science+Education%2fEquipment+and+Supplies%2fProteins+and+Proteomic+Studies%3a+Equipment+and+Reagents%2fPrecast+Polyacrylamide+Gels&catLevel=5&divName=Corporate&loggedIn=false&lang=English&country=HQ&catOID=-19481&isPA=false&serviceLevel=Lit+Request
2007-12-21 03:17:59 · answer #3 · answered by hcbiochem 7 · 3⤊ 0⤋
another stupid question. haha
2007-12-21 17:08:59 · answer #4 · answered by Der f2 member 1 · 0⤊ 1⤋