ELISA: Mutation can create a cutting site. Can mutation delete the cutting site also??

Question

I'm taking my first course of genetics in college.
In our lab, we conducted an ELISA experiment. After running on gel electropheresis, we observed one band from the gold standard. we had 3bands for non-tumor tissue from a cancer patient. we had 2bands for tumor tissue from the same patient and therefore we concluded that the patient was inherited the germ-line mutation and she or he developped a mutation during life time.
My understanding was that mutation can create a cutting site.
However I had another ELISA experiment that does not support this idea.
In this eperiment, we had 2bands for gold standard, 3bands for non-tumor tissues from a cancer patient. we also observed 1band from tumor tissue from the same patient. Can mutation delete a cutting site? I do not really understand how mutation can create and delete cutting sites. Depends on what kind mutation it is, results seem to differ. Can someone explain this to me please?

Answer 1

Think you chose the wrong forum, you probably need a scientific one. Sorry.

Answer 2

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