Why Liebermann-Buchard reagent will turn green in the presence of cholesterol?

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Why Liebermann-Buchard reagent will turn green in the presence of cholesterol?

2007-12-04 01:30:09 · 1 answers · asked by sheyunn1990 1 in Science & Mathematics ➔ Chemistry

1 answers

This test uses a chloroform solution of suspected cholesterol treated with acetic anhydride and sulfuric acid. Acetic anhydride acetylates the -OH ofcholesterol, and H2SO4 catalyzes a Friedel-Crafts addition of CHCl3 to the aromatic ring, producing a colored complex (blu, greenish-blue).

2007-12-04 01:53:15 · answer #1 · answered by steve_geo1 7 · 0⤊ 0⤋