During synthesis of RNA, which strand of DNA is used? I'm really confused. The promoter region is where the nucleotides ATG are. Is that on the 5' to 3' strand or 3' to 5' strand? Is only the strand with the promoter region involved? If RNA Polymerase adds complimentary nucleotides starting with the codon AUG, wouldn't that mean the opposite DNA strand of the "ATG" strand be used instead of the "ATG" strand?
So in nutshell, I'd like to know which strand of DNA is relevant during transcription and which strand of DNA the promoter is located on.
2007-11-29 16:15:12 · 4 answers · asked by Anonymous in Science & Mathematics ➔ Biology
always always always 5' to 3'......
2007-11-29 16:28:47 · answer #1 · answered by Franklin 7 · 0⤊ 0⤋
DNA bases are always added on in the 5' to 3' direction. Both strands can be transcribed but for each gene, it is the strand with the promotor on it that is used as the coding strand (the one where complementary bases will attatch to form the mRNA).
The other strand will be the template strand as it will be almost identicle to the mRNA strand formed except when Thymine is present in the DNA, Uraceil will be present in the mRNA
2007-11-30 03:32:06 · answer #2 · answered by giz 2 · 0⤊ 0⤋
5 to 3 strand.
both
2007-11-30 00:22:38 · answer #3 · answered by Anonymous · 0⤊ 0⤋
BOTH strands are duplicated, each in its 5' to 3' direction.
2007-11-30 01:43:09 · answer #4 · answered by Doctor J 7 · 0⤊ 0⤋