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We recently separated the pigments in a spinach leaf using chromatography with a solvent of 90% petroleum ether and 10% acetone solvent and our teacher wants us to compare our Rf values to standardized values that may or may not be published in books.

I've looked through some books on chromatography today at a library but couldn't find any values.

My question is, is there any specific book titles i should be looking for?

2007-11-02 15:17:53 · 2 answers · asked by fye 1 in Science & Mathematics Botany

2 answers

I didn't think this would be so hard to find, but I've spent the last 45 minutes trying to find a table with the Rf values instead of lab instructions to calculate the values. But here you go:

Edit - OK, I tried to put the website here, but it just goes to a Word Document. The table on the site says:
Pigment Color Rf value
carotene yellow-orange 0.91
chlorophyll a blue-green 0.63
chlorophyll b Green 0.58
xanthophylls Yellow 0.53
xanthophylls Yellow 0.47
xanthophylls Yellow 0.32

The source is:
"Thin Layer Chromatography of Vegetable Pigments"
Juniata College
Science in Motion Program

If you do a simple search for the title of the lab and put it in quotes, you can get to the whole article. Search "Thin Layer Chromatography of Vegetable Pigments"

OK - Now it's been an hour, but I hate to give up when I know something's out there!

2007-11-02 16:43:25 · answer #1 · answered by ecolink 7 · 2 0

Thin Layer Chromatography (TLC) is similar to Paper except rather than using a stationery phase of paper it uses a thin layer of adsorbent (silica gel or cellulose) on a flat, unreactive substrate. It is therefore better as it runs faster and has clearer separations

2016-05-27 03:10:05 · answer #2 · answered by ? 3 · 0 0

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