2007-10-11 07:55:19 · 5 answers · asked by Anonymous in Science & Mathematics ➔ Chemistry
Okay, but when running Indole tests, why am I testing for Indole... I know to see if it's present, but what relavence does it has to the bacterium? I'm completely confused.
2007-10-11 08:06:32 · update #1
With the test you proof the existence of the enzyme tryptophanase. If tryptophanase is available (e.g. in a bacterium) tryptophane will be decomposed to indole, pyruvic acid and ammonia. Indole forms with 4-(N,N-dimethylamino)benzaldeh y de (swearword?) the red cyanine dye Rosindol (german name).
If after addition a red color occurs, your bacterium is indole-positive.
greets
2007-10-11 09:30:12 · answer #1 · answered by pyrrol88 3 · 1⤊ 0⤋
What Is Indole
2017-01-20 11:08:51 · answer #2 · answered by Anonymous · 0⤊ 0⤋
Indole is a heterocyclic aromatic compound that has a pyrrole ring (5-membered ring with nitrogen) fused with a benzene ring. Take a look at the structure here:
ref: http://en.wikipedia.org/wiki/Indole
2007-10-11 08:00:55 · answer #3 · answered by Dr Dave P 7 · 1⤊ 0⤋
A compound obtained from coal tar and indigo and produced by decomposition of tryptophan in the intestine, where it contributes to the peculiar odor of feces. It is excreted in the urine in the form of indican. 3-Methyl-indole and L-tryptophan are implicated in causing acute interstitial pneumonia in cattle.
2007-10-11 08:04:01 · answer #4 · answered by Tenn Gal 6 · 1⤊ 0⤋
This is a compound whose structure is a benzene ring (hexagon) sharing a common side with a pentagon with one nitrogen instead of >CH in it. It is related to indigo, which is the blue dye for blue jeans. It is alsoa substituent of the amino acid tryptophan.
2007-10-11 08:01:32 · answer #5 · answered by steve_geo1 7 · 1⤊ 0⤋