2007-09-23 18:23:07 · 2 answers · asked by Hopeful 1 in Science & Mathematics ➔ Biology
It means empiracally determining the sequence of a specific gene this is usually done by isolating the gene (often by cloning it into a suitable vector) and then basically using chemical tags that terminate chain elongation at each base type (so a tag that stops A's being added, a tag that stops G's from being added, etc.). This used to be done old scool using radiolabeled and dideoxy-NTPs (they can't elongate) and running 4 different reactions then combining the data. Now we can use fluorescently labeled NTPs and run a sequencing reaction together.
2007-09-24 05:22:14 · answer #1 · answered by St. Judy's comet 3 · 0⤊ 0⤋
It means to list the bases that make up a gene, and keep the bases in order.
TACAAGGTCGTAATGATTACCA.......
2007-09-23 18:26:32 · answer #2 · answered by ecolink 7 · 0⤊ 0⤋