Why in blood count test, haemoglobin should not clump or should only a single layer in the result diagram?

Question

Add in : Why clumped haemoglobins show that the person is not healthy? A healthy person should see single layer of haemoglobin in the result picture taken under light microscope.

Answer 1

I think what you are refering to is the visualization of red blood cells on a blood film. In an appropriately prepaired blood film, the area where the blood cells are in a single layer are refered to as the "monolayer", as you travel further back into the slide to the point of origin where the original drop was placed to make it, it consists of a very thick area of clumping blood cells that is seldom used in the Laboratory, except for some specific instances. To see clumping or stacking of red blood cells in the monolayer, could be an indication of the presence of a cold agglutinin or rouleaux formation. Rouleaux can be present in diseases such as multiple myeloma, the cells appear as "stacks of coins". In normal blood films, we only use the monolayer to count cells because this uniform area of the slide provides the most accurate area of distribution of red cells and the most accurate area to evaluate the size and shape of the red blood cells in the smear. Hope this helps

Answer 2

It is actually the red blood cells that "clump" (each red blood cell contains nearly 300,000,000 hemoglobin molecules). Other whole blood components such as platelets also cause blood to "clump." This is why blood is collected in a tube containing an anticoagulant (e.g. EDTA).

When whole blood is spun in a centrifuge (at ~10,000 RPM X 5 minutes in a microhematocrit tube), the blood separates into red blood cells (on the bottom), and plasma (on top) with a small layer, called the buffy coat (composed of white blood cells) in the middle. You can actually see the division between the red cells and the plasma. The hematocrit is written as the percentage of red blood cells in a volume of whole blood.

EDTA, or ethylenediamine tetraacetic acid (1.5 mg/ml of blood), is the most commonly used anticoagulant in the test you are asking about. It prevents whole blood from "clumping" or coagulating after it has been drawn (otherwise red blood cells would "trap" other blood components, rendering the CBC results inaccurate).

Answer 3

Blood counts are expressed as a number per cubic millimeters. A clumped hemoglobin or several layers of cells irrationally alter the volume in relation to the number of cells.

Answer 4

You need to clarify your question because it does not make sense.

If you are asking, why doesn't the blood clot after being drawn, then I can help with that. After being drawn, the blood goes into a tube that has an anti-coagulant (different tests require different anti-coagulants so each color of tube is a different compound). Anyway, it mixes with the blood and prevents it from clotting.

Answer 5

i don't understand your question.