What happens to the staining? What will be the result? Expected acid fast organisms will not be acid fast? will ethyl alcohol interact with the mycolic acid of acid fast bacilli? Please explain your answer.
2007-06-29 15:17:29 · 3 answers · asked by Tapyboy 2 in Science & Mathematics ➔ Medicine
acid alcohol is used for decolorizing is actually for the non-acid fasgt so that they will not retain the carbolfuschin. if you use ethyl alcohol, even the non-acidfast will look acid fast becuase it is inadequate to remove the carbolfuschin to those that are not acid fast.
2007-06-30 07:20:22 · answer #1 · answered by Mike 3 · 1⤊ 1⤋
The walls of certain bacteria and parasites contain long chain(50-90 C atoms) fatty acids(Mycolic Acid), lending the property of resistance to decolorization of basic dyes by acid alcohol. Thus named Acid Fast Staining.
Ex. Mycobacterium tuberculosis with acid fast the surrounding will be colored blue but the organism will be colored red and either snapping or slipping.
So simply Acid Fast Organisms will be colored red
Non-Acid Fast Organisms will be colored blue
Ethyl alcohol will not interact with the mycolic acid and the organism will be decolorized.
2007-06-29 23:37:49 · answer #2 · answered by giftedman88 3 · 1⤊ 0⤋
What is acid alcohol?
2007-06-29 18:16:27 · answer #3 · answered by J.SWAMY I ఇ జ స్వామి 7 · 0⤊ 1⤋