It involves passing a mixture dissolved in a "mobile phase" through a stationary phase, which separates the analyte to be measured from other molecules in the mixture and allows it to be isolated.
For those new to separation science, an analogy which is sometimes useful is to suppose a mixture of bees and wasps passing over a flower bed. The bees would be more attracted to the flowers than the wasps, and would become separated from them. If one were to observe at a point past the flower bed, the wasps would pass first, followed by the bees. In this analogy, the bees and wasps represent the analytes to be separated, the flowers represent the stationary phase, and the mobile phase could be thought of as the air. The key to the separation is the differing affinities among analyte, stationary phase, and mobile phase. The observer could represent the detector used in some forms of analytical chromatography. A key point is that the detector need not be capable of discriminating between the analytes, since they have become separated before passing the detector.
2007-06-05 22:47:22
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answer #1
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answered by jsardi56 7
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It is used as a surface absorber from using a retarding system for compounds such as cellulose and various properties of silicone.
2007-06-06 04:44:14
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answer #2
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answered by Will 2
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