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how to prepare a buffer solution, 1L-volume, 0.05-Concentration, pH-7.4

2007-06-05 09:02:34 · 3 answers · asked by w girl. 1 in Science & Mathematics Chemistry

3 answers

Weigh out 0.05 moles of KH2PO4 and dissolve it in about 800 mL of water. Using a pH meter raise the pH of the solution to 7.4 by slowly adding KOH to the solution. When the pH is 7.4, add water to give a final volume of 1.0 L.

When you dissolve KH2PO4, the pH of the solution will be less than 7, and so you can simply add KOH to neutralize the H2PO4- ions, converting them to HPO4^2- ions.

Alternatively, you could make two solutions: 0.05 M KH2PO4 and 0.05 M K2HPO4 and pour them together until the pH is 7.4. The end result will be the same either way.

2007-06-05 09:10:00 · answer #1 · answered by hcbiochem 7 · 0 0

Mysterious

2016-04-01 03:59:15 · answer #2 · answered by Anonymous · 0 0

http://www.chemtutor.com
K3PO4

2007-06-05 09:08:19 · answer #3 · answered by Anonymous · 0 1

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