who can explain principle and result of osmosis fragility curve?

Question

the purpose of this test is to test the osmotic resistance of red blood cell using different type of solution such as sodium chloride of different concentration, sucrose solution and urea solution. at the end of test, we are asked to determine the percentage of red blood cell hemolysis..

Answer 1

The curve describes the ease with which red cells undergo lysis (bursting) in saline solutions. According to the reference below, osmotic fragility is defined as "the ease with which the cells burst in hypotonic solutions and is expressed in terms of the concentration of the saline solution in which the cells are hemolyzed." (A lower concentration implies more fragility.) Ease of bursting is a function of the ratio of cell surface area to volume. The greater this ratio, the more rapidly the cell takes in enough water to burst.