what are the difference between single digestion and double digestion about restriction enzymes?

Question

what are the advantage of single digestion

Answer 1

Restriction enzymes (endonucleases) are used for a variety of reasons in molecular genetics, including obtaining a "map" and cloning DNA.

Single digests consitute DNA being treated with one restriction endonuclease, whereas double digests contain 2 enzymes.

At times, it is difficult (or not possible) to perform double digests ... especially when the 2 enzymes have very different requirements for their activities (e.g. salt concentration, temperature optimums, ...).

If a DNA restriction map is known for a particular enzyme, and if the DNA is treated with this enzyme, then one can ascertain whether the digest was complete or not. However, if a restrictioin map is just being compiled, and if the DNA is treated with 2 enzymes in a double digest, at times difficulties may arise in determining the map if either (or both) enzymes did not completely digest the DNA.

Answer 2

Double Digestion

Answer 3

This Site Might Help You.

RE:
what are the difference between single digestion and double digestion about restriction enzymes?
what are the advantage of single digestion

Answer 4

Single digestions are performed using a single restriction endonuclease and double digests are performed using two enzymes at the same time. Digestions are routinely used in molecular cloning for a variety of reasons. Double digests are often used for determining orientation of a cloned insert or for mapping, while single digests are often used for very basic cloning, although double digests might also be used for this purpose.

Answer 5

Education is good, because it teaches you how to learn, how to discipline your mind. Knowledge for a Christian is knowing the word, knowing your God, His nature, His will, His outlook on things. Wisdom is looking at things from God's perspective and putting them into action. Knowledge is great, but too much of it can puff you up if you don't counteract it with fruit of the Spirit. Ever know a person who's very knowledgeable, but has no temperance and no love? Their knowledge is almost useless in the long run. But wisdom? You find that and your soul shall live, because when you find wisdom, you find the way God Himself looks at a situation. Proverbs 3: By wisdom the Lord laid the earth’s foundations, by understanding he set the heavens in place; by his knowledge the watery depths were divided, and the clouds let drop the dew.