what are the steps for protein synthesis?

Answer 1

Transcription: DNA is too large to leave the nucleus. If the program it contains must leave the nucleus, an intermediate must be produced. This intermediate is called m-RNA. m-RNA is formed in the following way, RNA polymerase binds to, a region of the DNA called, the promoter. Here is found the start codon TAC which codes for the amino acid methionine. Once this has been established the m-RNA sequence is produced growing from the 5' - 3' region. This occurs at the rate of 60 nucleotides / sec. The terminator sequence will keep the process from going on indefinitely. The eukaryotic m-RNA must be modified before going to the ribosome, while the prokaryotic does not.

Translation: t-RNA : a specialized form of RNA used to carry a specific amino acid to the ribosome and place it in the proper position. t-RNA contains 80 nucleotides in the form of a clover leaf. The t-RNA must attach the correct amino acid to itself. The process is as follows:

1. The specific amino acid reacts with a specific enzyme and ATP to release 2 P and combine the AMP and the amino acid.

2. The t-RNA will replace the AMP and bond with the amino acid.

Ribosomes: Before translation ribosomes consist of 2 separate sub-units. As translation proceeds the ribosome parts come together to allow protein synthesis to begin.

There are 3 steps to protein synthesis:

1. Initiation: m-RNA binds to the small subunit of the ribosome. There is 1 binding site for the m-RNA, and 2 binding sites for the t-RNA. The P site holds for the t-RNA carrying the growing polypeptide and the A site for the t-RNA carrying the next amino acid. AUG ( start codon ) is in place and the appropriate t-RNA delivers the amino acid Methionine. The large sub unit then joins and creates a functional ribosome.

2. Elongation: The chain moves from the 5' - 3' direction caused by the use of GTP and several protein-enzyme complexes. As the polypeptide chain is produced the t-RNA move from site A to site P, and then are released into the cytoplasm. The polypeptide chain continues to grow, and is held to the ribosome by the t-RNA at the P site.

3. Termination: The termination codons: UAA, UAG, and UGA stop the process by adding water to the end of the chain instead of an amino acid. In order for the protein to become functional, it must be folded into its appropriate shape,and/ or have some amino acids removed, and/or have some of the polypeptides modified by adding sugars or phosphate groups to them.

The Wobble Phenomenon: There are only 40 different types of t-RNA and 64 codons. This means that some of the t-RNA can pair up with several different codons. This can occur because the third base of a t-RNA molecule can form a hydrogen bond with more than one kind of base. U in the third position can bond with A or G in the corresponding position.

Much of the DNA does not code for the protein. Some eukaryotic DNA has long non coding portions called Introns. The coded portions of the DNA are called exons. Before the m-RNA leaves the nucleus the introns are cut out and the exons are spliced together and can now carry on its function.

Answer 2

Chemical or biological?

Chemically, you form proteins by adding amino acids through condensation reactions

Biologically, proteins are formed by translation of messenger RNA.

Answer 3

in short...
gene->messenger rna(initiation,elongation,termination)->protien(capping of 5' end,polyadenylation,splicing)
in nature

Answer 4

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