2007-03-10 04:26:30 · 3 answers · asked by nika a 1 in Health ➔ Diseases & Conditions ➔ Infectious Diseases
You construct an RNA primer that is complementary to the three prime end of the HIV RNA. Then, to your HIV RNA, you add the primer, some oligonucleotides and a RNA dependant RNA polymerase (some viruses have these to replicate their RNA genomes). Sit back and watch whilest you get your antisense oligonucleotide RNA.
To purify, you can run it down a chromatagraph coloum, with beads coated with either HIV RNA antibodies, or fragments of HIV RNA.
Edit: Its a slight variation on the standard PCR, which normally is used for DNA.
2007-03-10 05:46:57 · answer #1 · answered by Bacteria Boy 4 · 0⤊ 0⤋
There is a special database of HIV Genome sequences. Somewhat easier to find HIV genomes there, than in GenBank.
2007-03-12 14:22:55 · answer #2 · answered by Phillip D 2 · 0⤊ 0⤋
hey..i find this interesting...will read abt it and let u know
2007-03-10 12:32:00 · answer #3 · answered by curiousme2006 2 · 0⤊ 0⤋