On the Maconkey plate, i saw red colonies and clear colonies and on the Hektoen plate i saw yellowish-orange colonies and colonies with black centers (H2S) production. But when i tried to isolate them on the Hek plate again, I cannot see growth from the black dotted colonies at all, while there was the same growth of orange-red colonies from the other organism. Is it possible that the black dotted colonies can disappear? i'm just confused; i've done the gram stains and both are gram neg. rods while the reddish-orange colonies resembles dipplo-cocci looking rods and the clear black dotted colonies had a small thin gram neg rod with majority of short rods. Oxidase test was done on both, and both are oxidase neg. Any helps to what i should focus on as to their identities? I know that one is a glucose fermenting organism while the other, i'm just not sure since i wasnt able to do any other chemical tests on it yet. i just want to have an idea of where i should be headed.
2007-02-24 19:04:24 · 2 answers · asked by Anonymous in Science & Mathematics ➔ Biology
ok, i just had results for some other tests that were available: first organism with the purple-pink colonies in the MAC plate had A/A on the DSIA, + citrate, -urease, nonmotile,+indole -H2S. The 2nd organism with clearish brown colonies on the MAC plate is K/A for DSIA, H2S+, motile, citrate -, urease -, indole-, So any leads? I think the 1st organism is Klebsiella while the other is Salmonella.
2007-02-28 05:42:32 · update #1
I can't tell you what you have, but I can caution you to only trust the results of a MaConkey plate when they're fresh out of the incubator. If you stick them in the frig for awhile, they all wind up looking the same because the indicator in MaConkey is soluble.
Also be sure to wash your hands really well with soap after handling those samples. If they're what you think they are they will both make you miserably sick and cause you to frequent the bathroom.
2007-02-28 17:22:30 · answer #1 · answered by BP 7 · 0⤊ 0⤋
first organism sounds like kielbasa and the second sounds like your playing with salmonella.need more data.
2007-03-04 18:13:35 · answer #2 · answered by endgame1915 3 · 0⤊ 0⤋