Transcription homework help (Bio 1)?

Question

- Which side of the DNA double helix is used as the template?
- What happens to the RNA strand after it reaches the remination signal on the DNA template?
- What happens to the DNA strand after the new RNA strand is finished?

Thanks a lot.

Answer 1

As in DNA replication, transcription proceeds in the 5' → 3' direction (ie the old polymer is read in the 3' → 5' direction and the new, complementary fragments are generated in the 5' → 3' direction)

RNA polymerase (RNAP) recognizes and specifically binds to the promoter region on DNA. At this stage, the DNA is double-stranded ("closed"). This RNAP/wound-DNA structure is referred to as the closed complex.
The DNA is unwound and becomes single-stranded ("open") in the vicinity of the initiation site (defined as +1). This RNAP/unwound-DNA structure is called the open complex.


Intrinsic termination (also called Rho-independent termination) involves terminator sequences within the RNA that signal the RNA polymerase to stop. The terminator sequence is usually a palindromic sequence that forms a stem-loop hairpin structure that leads to the dissociation of the RNAP from the DNA template
Rho-dependent termination uses a termination factor called ρ factor(rho factor) to stop RNA synthesis at specific sites. This protein binds and runs along the mRNA towards the RNAP. When ρ-factor reaches the RNAP, it causes RNAP to dissociate from the DNA, terminating transcription.

The DNA on the template strand between the +1 site and the terminator is transcribed into RNA, which is then translated into protein.

Answer 2

pff, i presumed i understood genetics fairly properly yet i actually could think of roughly those. i won't be able to guarantee my anwser's are superb suited yet i will attempt. a million. i'm not sure what you mean with the aid of chromosome shape, yet what i leared approximately genetic expression is that some alleles are dominant over recessive ones, meaning the dominant alleles may be the version we see in a individual, it rather is the version of a definite gene it rather is being expressed. now no longer all genes are expressed in each and each cellular. 2 varieties: introns and extrons, i think of to recollect the extrons are those no longer being expressed, they get decrease out while mRNA is being produced. this preocess is "arranged" with the aid of a mastergen, yet i do no longer understand precisely the way it works, nor if the form of the chromosome has something to do with it, i assume that is all related such that the form of the chromosome relies upon on which genes are on that chormosome.... I have no thought approximately the different questions by any means. sorry to no longer have the means to be of extra help....