If a Gram stained a sample from a pure culture of bacteria and observed red and purple cocci.?

Question

Suppose you Gram stained a sample from a pure culture of bacteria and observed a field of red and purple cocci. Adjacent cells were not always the same color. What would you conclude?

Answer 1

I think that the hint in this question is that adjacent cells are not always the same colour. The easy answer is a poorly performed or uneven Gram stain. However, the fact that adjacent cells aren't the makes me think that that's not the problem.

Sometimes the cell wall of aging Gram positive cells starts to degenerate or break down a little. This results in them sometimes staining negative. So my guess would be that the culture was old, so it contained a mixture of young and very old cells, giving a mix of positive and negative staining cells.

Answer 2

Gram unfavourable is often crimson/crimson so i could end they're gram beneficial and your inocculating loop wasnt suitable sterilized! yet I certainly have examine that streptococci could nicely be the two gram + and gram -. wish this helps!

Answer 3

I believe ur work is contaminated.