ELISA technique of Aspergillus in ration?

Answer 1

ELISA is a way of detecting something in a biological system (Enzyme Linked ImmunoSorbent Assay).

If you want to detect Aspergillus, you use an antibody that recognizes the Aspergillus. You get a plate coated with this antibody and then expose it to the patient's blood/body fluids/whatever.

Then you wash the plate so the excess is removed and only the antibody-antigen (ie Aspergillus) combinations remain.

Then you apply another antibody that recognizes the these complexes and is bound to an enzyme. If there is any aspergillus antigen then it will have been bound to the plate. Wash off the excess so there is no enzyme left except that bound to aspergillus antigen.

Then you expose the plate to the indicator - something that the enzyme reacts with to change colour or whatever.

If there is a colour change then this means the enzyme is present. This in turn means that the antibody-antigen complexes are present and that finally means the antigen (ie Aspergillus) is present.

I'm not sure what you mean by "in ration" - are you looking for Aspergillus in food?