how to stain and see stomata?

Answer 1

Eosin is most often used as a counterstain to haematoxylin, imparting a pink or red colour to cytoplasmic material, cell membranes, and some extracellular structures. It also imparts a strong red colour to red blood cells. Eosin may also be used as a counterstain in some variants of Gram staining, and in many other protocols. There are actually two very closely related compounds commonly referred to as eosin. Most often used is eosin Y (also known as eosin Y ws or eosin yellowish); it has a very slightly yellowish cast. The other eosin compound is eosin B (eosin bluish or imperial red); it has a very faint bluish cast. The two dyes are interchangeable, and the use of one or the other is more a matter of preference and tradition.

Procedure- The peeling of a leaf is kept in dilute aquaous solution of Eosin for a few minutes and the washed with water and mounted in either water or glycerene.

The stomata ( i.e. Guard cells are stained pink or red) are easily seen.

Answer 2

Use chromatin maybe. It is used to see vessels in the stem by staining it, should most probably work for stomata as well. Normally when you see pictures from different types of microscpoes in text books they are purple in colour and it must be because they have been stained with chromatin, therefore I would be most suitable to use chromatin.

Answer 3

stomata of fresh material stain selectively with very dilute aqueous solutions of neutral red and toluidine blue.. I did that for my lab

Answer 4

I used methylene blue in botany lab