what are southern and western screening tecniques?

Question

it is apart of selection and screening techniques in rdna technology

Answer 1

Southern analysis is the use of a DNA probe of known sequence, labelled with a radioactive or flourescent marker. The probe is complimentary to a sequence of interest, often a gene. To determine if a given DNA sample has the sequence of interest the DNA sample is separated on an agarose gel, denatured, transfered to a membrane, then the probe is annealed.

Western analysis is the use of antibodies to determine if a protein of interest is present. Just like the Southern blot, the protein sample in question is separated on a gel, transferred to a membrane, then probed with the labelled antibody.

Answer 2

southern blotting
introduction
lifesciences.asu.edu/resources/mamajis/southern/southern.html
technique
en.wikipedia.org/wiki/Southern_blot
western blottin
introduction
lifesciences.asu.edu/resources/mamajis/western/western.html
technique
en.wikipedia.org/wiki/Western_blot