2006-11-11 20:23:25 · 7 answers · asked by *** 2 in Science & Mathematics ➔ Botany
HPLC originally stood for High Pressure Liquid Chromatography, but as techniques changed and extremely high pressures were not needed, the name was changed to High Performance Liquid Chromatography.
HPLC is a form of column chromatography where very small columns filled with very small sized stationary phase materials is eluted with a mobile phase which is pumped through the column.
A pressure lock system is required to inject a small sample (often measured in microliters) onto the "top" of the column.
The amount of chemical absorbtion onto the stationary phase relative to the solubility in the mobile phase will cause different substances to move through the column at different rates (usually called the retention time for the substance).
2006-11-11 20:51:01 · answer #1 · answered by Richard 7 · 73⤊ 0⤋
HPLC originally stood for High Pressure Liquid Chromatography, but as techniques changed and extremely high pressures were not needed, the name was changed to High Performance Liquid Chromatography.
HPLC is a form of column chromatography where very small columns filled with very small sized stationary phase materials is eluted with a mobile phase which is pumped through the column.
A pressure lock system is required to inject a small sample (often measured in microliters) onto the "top" of the column.
The amount of chemical absorbtion onto the stationary phase relative to the solubility in the mobile phase will cause different substances to move through the column at different rates (usually called the retention time for the substance). All the other people that said something but this are wrong. Hope this helps!
2006-11-12 12:03:42 · answer #2 · answered by SCSA 5 · 0⤊ 0⤋
High Performance Liquid Chromatography (HPLC):
HPLC is a popular method of analysis because it is easy to learn and use and is not limited by the volatility or stability of the sample compound.
Check out the site as well
Greetz
2006-11-11 20:35:32 · answer #3 · answered by lolkedouma 2 · 1⤊ 0⤋
High performance liquid chromatography or high pressure liquid chromatography
it puts liquids under huge pressure and run it in a column. The pressure helps seperate the various chemicals in the liquid. Its usually used to analyze chemicals.
http://en.wikipedia.org/wiki/HPLC
2006-11-11 20:36:14 · answer #4 · answered by Anonymous · 2⤊ 0⤋
Annie's method is pretty good - apart from one thing. If you are using aspirin that you have synthesized yourself, then remember that it is likely to contain some unreacted salycilic acid, so when you hydrolyse the "aspirin" sample and then read the "salicylic acid" concentration from the calibration graph, what you are actually measuring is the TOTAL salycilic acid concentration : the amount from the hydrolysis of your aspirin sample plus the amount of salycilic acid present as a contaminant! That's why the answer is often greater than 100%. You can work out the original amount of aspirin by calculation after this. Good luck!
2016-05-22 06:43:09 · answer #5 · answered by Anonymous · 0⤊ 0⤋
High performance liquid chromatography or high pressure liquid chromatography.
generally it is used for separation of pigments.
2006-11-11 22:38:22 · answer #6 · answered by Ishika 1 · 0⤊ 0⤋
high performance liquid chromatography...it's a method of separating components of a compound...that's all i can share...try wikipedia...
2006-11-12 09:41:43 · answer #7 · answered by Treat 2 · 0⤊ 1⤋