2006-09-27 03:16:44 · 2 answers · asked by aditya n 1 in Science & Mathematics ➔ Medicine
The Romanowsky stains are a series of Blue/Red stains where the blue binds to acidic substances and the red binds to neutral or basic substances in cells. They were developed by Romanowsky in the late 1800s and people use this name to group these stains as others added their names to modifications of this simple staining procedure so you will see names like "Wright's" Stain, Giemsa Stain etc. Because of the ability of these stains to show differences in coloration and size and shape of nucleus and cytoplasm, they have become useful in differentiating different cell types. They became the main way the clinical laboratory test called the "differential count" was done before development of light scatter and fluorescence activating cell sorting made this an automated procedure. As a back up, stained blood smears are routinely made and examined if the automated test shows abnormalities. See the winkepedia info on this.
2006-09-27 03:33:25 · answer #1 · answered by Art 3 · 1⤊ 0⤋
Romanowsky Stain
2016-11-09 23:27:21 · answer #2 · answered by nickelston 4 · 0⤊ 0⤋
Romanowsky staining was a prototypical staining technique that was the forerunner of several distinct but similar methods, including Giemsa, Jenner, Wright, and Leishman stains, which are used to differentiate cells in pathologic specimens.
Ehrlich had used mixtures of acidic and basic dyes for this purpose in 1879: in 1891 Romanowsky and Malakowsky independently developed a technique using a mixture of Eosin Y and oxidated Methylene Blue that was also useful for this purpose. Because the aqueous dye solutions were unstable, methanol was introduced as a solvent, and Leishman (in 1901) and Wright (in 1902) advocated use of methanol as a fixative prior to staining. Giemsa in 1902 improved this technique by standardizing the dye solutions and adding glycerol to increase solubility and stability.
The oxidation of Methylene Blue in aqueous solution using heat and alkali produces a mixture of Azure A, Azure B, Methylene Violet and Methylene Blue. Eosin Y is then added to produce a "neutral" dye. The precipitate is then dissolved in a mixture of methanol and glycerol to form a stock solution: this is diluted with water or an aqueous buffer to form a working solution that is used in the preparation of pathology specimens
2006-09-27 04:49:19 · answer #3 · answered by Anonymous · 2⤊ 0⤋
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RE:
what are the romanowsky stains?
2015-08-19 05:43:52 · answer #4 · answered by Racheal 1 · 0⤊ 0⤋
are you talking about gram staining?
2016-03-18 21:49:50 · answer #5 · answered by Carla 2 · 0⤊ 0⤋