Why is a mass spectrometer often used in conjuction with a gas chromatograph machine?

Answer 1

Because the CG is cheaper (both running and initial price) and actually works better on the lighter elements. MS is great for transition metels, but it is not that great with H, He, etc.

Answer 2

Think of the mass spectrometer as a powerful detector. The components of a mixture will have been separated by the chromatography. Each component will then analysed to produce its characteristic mass spectrum.

Answer 3

because mass and gas go to gather. there can never be less of either.

Answer 4

Noun 1. mass spectrometer - spectroscope for obtaining a mass spectrum by deflecting ions into a thin slit and measuring the ion current with an electrometer
Synonyms: spectrometer

By Wordnet Dictionary




Mass spectrometry is a technique for separating ions by their mass-to-charge (m/z) ratios.

Mass spectrometry can be divided into two broad applications:

1) identification of compounds by the mass of one or more elements in a compound
2) determination of the isotopic composition of one or more elements in a compound

The most common form of mass spectrometry is gas chromatography-mass spectroscopy (GC/MS). In this technique a gas chromatograph is used to separate compounds. This stream is fed into the ion source, a metallic filament to which voltage is applied. This filament discharges electrons which ionizes compounds. The ions can then further fragment, yielding predictable patterns. The stream then passes into the detector.

For large molecules typical of biological applications, special techniques are used. The ion source subjects a sample of material to an electrical charge that causes the material to be ionized. The ions are then transported by magnetic or electrical fields to the mass analyzer. Types of ion sources include electrospray ionization, chemical ionization, fast atom bombardment, matrix-assisted laser desorption ionization, Thermal Ionisation (TIMS) , Secondary Ionisation (SIMS) and Plasma Source.

Atmospheric Pressure chemical Ionization (APcI), an adaption of chemical ionization allows for high flow rates typical of HPLC to be used directly, often without diverting the larger fraction of volume to waste. Typically these mobile phase / analyte sytems are heated to temperatures in excess of 400 degrees Celsius, sprayed with high flow rates of nitrogen and the entire aerosol cloud is subjected to a corona discharge that creates ions. The term "chemical" ionization comes from the fact that the initial ions produced are typically those of the mobile phase and its modifiers. The analyte is ionized by charge transfer during collisions in the high (atmospheric) pressure region of the outer source.

The mass analyzer is the most flexible part of the mass spectrometer. Since an electric or magnetic field can deflect charged particles, and since the kinetic energy imparted by motion through an electric field gives the particles an inertia dependent on the particle's mass, the mass analyzer uses these facts to steer certain masses to the detector based on their mass-to-charge ratios (m/z) by varying the electrical or magnetic field. It can be used to select a narrow range of m/z or to scan through a range of m/z to catalog the ions present. Besides the original magnetic-sector types, several types are currently in more common use, including time-of-flight, ion trap, and quadrupole mass analyzers.

The detector records the charge induced when an ion passes by or hits a surface. If a scan is conducted in the mass analyzer, the charge induced in the detector during the course of the scan will produce a mass spectrum, a record of the m/z's at which ions are present.

A tandem mass spectrometer is one that is capable of multiple rounds of mass spectrometry. For example, one mass analyzer can isolate one peptide from many entering a mass spectrometer. A second mass analyzer then stabilizes the peptide ions while gas collides with them, causing them to fragment. A third mass analyzer then catalogs the fragments produced from the peptides. This process, called collision induced dissociation, is the basis of many experiments in proteomics.

Mass spectrometry is also used to determine the isotopic composition of elements within a sample. Differences in mass among isotopes of an element are very small, and the less abundant isotopes of an element are typically very rare, so a very sensitive instrument is required. These instruments are called isotope ratio monitoring mass spectrometers (irm-MS) and use a single magnet to bend a beam of ionized particles towards a series of cups which convert particle impacts to current.


A mass spectrometer is a device designed to produce a mass spectrum of a sample to find out its composition. This is normally achieved by ionizing the sample and separating ions of differing masses and recording their relative abundance by measuring intensities of ion flux. A typical mass spectrometer comprises three parts: an ion source, a mass analyzer, and a detector. Mass spectroscopy allows detection of compounds by separating ions by their unique mass.

Techniques for ionization have been key to determining what types of samples can be analyzed by mass spectrometry. Two techniques which are often used with biological samples include electrospray ionization (for which John Fenn received the Nobel Prize in Chemistry in 2002) and Matrix-assisted Laser Desorption Ionization (MALDI) (for which Koichi Tanaka received the Nobel prize the same year). In both cases, Wilkinson ions are produced which can then enter the mass analyzer.

Mass analyzers of many varieties have been produced. Perhaps the easiest to conceptualize is the Time-of-flight (TOF) analyzer, typically integrated with MALDI ion sources. Ions are boosted to the same kinetic energy by passage through an electric field, and the times they take to reach the detector are measured. The smallest ions (or those most highly charged) move most rapidly. Quadrupoles and quadrupole ion traps use electrical fields to selectively stabilize or destabilize ions falling within a narrow window of m/z values. Sector instruments change the direction ions are flying through the mass analyzer. Fourier Transform Mass Spectrometry uses perturbations in magnetic fields to measure m/z values extremely accurately. The best mass analyzer for an experiment depends upon the type of information to be gleaned from the experiment.

The final element of the mass spectrometer is the detector. Typically, some type of electron multiplier is used, though others (such as Faraday cups) have been employed. Because the number of ions leaving the mass analyzer at a particular instant is typically quite small, significant amplification is necessary to get a signal.


History

The first mass spectography technique was described in an 1899 article by English scientist J.J. Thomson. The processes that more directly gave rise to the modern version were devised by A.J. Dempster and F.W. Aston in 1918 and 1919 respectively.