Iwanna do a smalll scale project on phage therapy for MRSA.Can anyone suggest the way to do it.for eg. where to get MRSA strains from?
2006-09-15 01:20:24 · 5 answers · asked by sanjana 1 in Science & Mathematics ➔ Medicine
If you are not associated with a microbiological lab that can obtain these strains for you and set up the proper quality and safety controls then you should leave this to people who can do meaningful research. If you are associated with such a lab or university, then ask around. It's fairly simple. In fact, it may be too simple. There are ways of obtaining isolated MRSA and identifying the strains yourself, but I pray that this is illegal and that such cultures are handled by conscientious people.
Tell me more about your project and maybe I can help.
2006-09-15 01:58:49 · answer #1 · answered by Nick â? 5 · 5⤊ 0⤋
pretty sure that they don't just hand out MRSA to anyone who asks. do you have bsl2 or possibly bsl3 facilities to work in?
the atcc sells ~200 staph aureus strains, perhaps one of those might fit your qualifications, but they have restrictions on who samples are sent to, and where they're allowed to be sent, for a reason.
for your experiment, i don't believe that there is any need to actually use a MRSA, the bacteriophage typically target some aspect of the cell that's required for viability, and the topics topics aren't necessariliy connected.
it would be plausible for you to get Staph aureus (start swabbing people's skin) and try your phage experiments with that. then, IF, you find phage capable of lysing the bacteria, perhaps you could try and get in contact with a facility that's equipped to handle MRSA. or set up such a collaboration first.
it would also be worth your time to see if what you're planning on testing out has been patented or published already.
2006-09-15 13:23:16 · answer #2 · answered by John V 4 · 0⤊ 0⤋
You will be able to get plenty of MRSA strains from your local hospital, particularly any hospital with an intensive care unit (ICU) however your project will have to meet the criteria of the hospital ethic committee before they will participate.
Giving you a strain of multi-resistant bacteria would certainly count as participation.
2006-09-15 08:35:48 · answer #3 · answered by Orinoco 7 · 0⤊ 0⤋
Any pustule from a patient with bed sores must supply Staph resistant to all antibiotics.
2006-09-15 08:45:10 · answer #4 · answered by J.SWAMY I ఇ జ స్వామి 7 · 0⤊ 0⤋
why don´t you try to cultiv them 1st in nutritiv agar ??
2006-09-15 08:34:09 · answer #5 · answered by Markitos 1 · 0⤊ 0⤋