WHAT ARE THE VARIOUS DEFINITIONS OF DNa RECOMBINATIONS AND WHAT ARE THE SALIENT FEATURES OF THIS CONCEPT?

Question

WHAT ARE THE VARIOUS DEFINITIONS OF DNa RECOMBINATIONS AND WHAT ARE THE SALIENT FEATURES OF THIS CONCEPT

Answer 1

Recombinant DNA (sometimes rDNA) is an artificial DNA sequence resulting from the combining of two other DNA sequences in a plasmid. Recombinant proteins are proteins that are produced by different genetically modified organisms following insertion of the relevant DNA into their genome. As this recombines the DNA of two different organisms, the word recombinant is used to refer to this process. Recombinant DNA technique was discovered by Stanley Cohen and Herbert Boyer in 1973, Nov 1973 publication of “Construction of Biologically Functional Bacterial Plasmids in vitro”, this paper described a technique to isolate and amplify genes, or DNA segments, and insert them into another cell with precision. Recombinant DNA technology was made possible by the discovery of restriction endonucleases by Werner Arber, Daniel Nathans, and Hamilton Smith, for which they received the 1978 Nobel Prize in Medicine.

The term recombinant DNA refers to a new combination of DNA molecules that are not found together naturally. Although processes such as crossing over technically produce recombinant DNA, the term is generally reserved for DNA produced by joining molecules derived from different biological sources.

Answer 2

Recombinant DNA and recombination are different.

Recombination, also known as genetic recombination or crossing over- is a process for instance during meiosis where at the pachytene stage maternal and paternal chromosomes exchange genetic material. recombination can also be intra allelic process where for inststance it is though Telomere rapid deletion events occur via the invasion of the 3' single stranded telomere end into the upstream telomere (composed of TTAGGG repeats), results in a holiday junction, and subsequent loss of the sequence downstream of the junction