which all organs are affected and how in Acute lymphoblastic leukemia?

Answer 1

ALL, generally effects the bone marrow. Bone marrow is the spongey stuff inside your bones. What it does, is it makes your blood; red cells( transport of food and air to your cells so they can live), white blood cells( Your bodies defenses), platelet and factors (forms clots- protection from blood loss). ALL, effects the White blood cell production. Cancer is defined as one cell or type of cell that replicates so frequently that it takes over the whole system, sometimes forming lumps, but in this case, It is a type of cell that forms your lymphocytes. There are five kinds of white blood cells and they each tackle different types of invaders- they are specialized soldiers in the defense of your body. Anyway... the lymphocyte parent cell, is rapidly making blasts... the youngest kind of lymphs. It makes so many of them, that instead of staying inside the bone marrow and maturing, they get pushed out into the blood. Well, when this happens, they haven't had the trainning (maturity) they need to fight off colds and the flu and stuff, and anything it encounters will kill it. It basically will leave you with no immune system, because it crowds out production of all other white blood cells and all you have are these too young of cells to defend you. Your body cannot fight off anything. Even normal household germs that are on your skin, and there are some and they normally cause you no harm what so ever, they will start to with this disease. So, the only organ effected by the disease is your bone marrow. Unless it spreads... and that is too unpredictable to discuss. Treatments may effect organs, each treatment is different. Your doctor will consult you on treatments when it come to that section.

Answer 2

1

Answer 3

I am going to give a short answer: ALL is a cancer of a particular type of white blood cell. These cells are made in the bone marrow and the bone marrow goes wild making tons of these immature WBCs - so much so that it does not make many of the other kinds of red blood cells, white blood cells or platelets. So the patient may be anemic, or susceptible to infection, or susceptible to bleeding - possibly nose bleeds or excess bruising because of the lack of platelets. The excess leukemic cells may be released in the blood circulation, or they may end up "infiltrating" or filling up other organs like the liver, spleen or lungs. If in the spleen or liver - these organs are enlarged and hard. If in the lungs the patient may have pneumonia symptoms.

Answer 4

The bone marrow is replaced by abnormal blast cells. They infiltrate lymph nodes, spleen and other organs including the brain at times.

Answer 5

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Answer 6

2

Answer 7

Acute Lymphoblastic Leukemia
Last Updated: July 11, 2006 Rate this Article
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Synonyms and related keywords: acute lymphocytic leukemia, acute lymphatic leukemia, acute lymphoid leukemia, ALL, pediatric cancer, childhood cancer, childhood malignancy, inherited genetic syndromes, lymphoblastic leukemia, leukemia, leukemic blasts, T cell, T-cell ALL, B cell, B-lineage ALL, BCR-ABL, MLL, high-risk ALL, exposure to ionizing radiation, exposure to electromagnetic fields, allogeneic hematopoietic stem cell transplantation, HSCT, bone marrow failure, anemia, thrombocytopenia, neutropenia, petechiae, bleeding, lymphadenopathy, hepatosplenomegaly, bone pain


INTRODUCTION Section 2 of 11
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Background: Acute lymphoblastic leukemia (ALL) is the most common malignancy diagnosed in children, representing nearly one third of all pediatric cancers. The annual incidence of ALL is about 30 cases per million people, with a peak incidence in children aged 2-5 years. Although a few cases are associated with inherited genetic syndromes, the cause of ALL remains largely unknown.

Many environmental factors (eg, exposure to ionizing radiation and electromagnetic fields, parental use of alcohol and tobacco) have been investigated as potential risk factors, but none has been definitively shown to cause lymphoblastic leukemia. Improvements in diagnosis and treatment have produced cure rates that now exceed 80%.

Further refinements in therapy, including the use of risk-adapted treatment protocols, may improve cure rates for patients at high risk while limiting the toxicity of therapy for patients with a low risk of relapse (see Risk classification). This article summarizes advances in the diagnosis and treatment of childhood ALL.


Pathophysiology: In ALL, a lymphoid progenitor cell becomes genetically altered and subsequently undergoes dysregulated proliferation and clonal expansion. In most cases, the pathophysiology of transformed lymphoid cells reflects the altered expression of genes whose products contribute to the normal development of B cells and T cells.

Leukemic blasts have long been thought to represent the clonal expansion of hematopoietic progenitors blocked during their differentiation at discrete stages of development. Recent data challenge this theory and suggest that leukemia arises from the stem cell that acquires features of differentiated cells. Although this observation may appear to be a subtle difference, it is important because it implies the need to eradicate the leukemic stem cell, and not just the differentiated blasts, to achieve a cure. Nevertheless, leukemic blasts provide large, uniform populations of cells for molecular and functional analyses.

ALL is generally thought to arise in the bone marrow, but leukemic blasts may be systemically present at the time of presentation. They may be present in the bone marrow, thymus, liver, spleen, lymph nodes, testes, and CNS.


Frequency:


In the US: Each year, 2000-2500 new cases of childhood ALL are diagnosed.
Internationally: Throughout the world, the incidence rate is thought to be similar to that in the United States.
Mortality/Morbidity: Despite overall improvements in outcome, the prognosis for patients whose leukemic blast cells carry the BCR-ABL fusion created by t(9;22) or the MLL genetic rearrangements created by translocations involving 11q23 is poor. Their estimated event-free survival (EFS) is only about 30%. Until recently, allogeneic hematopoietic stem-cell transplantation (HSCT) during the first remission was believed to be the only curative treatment option for these 2 groups of patients.

However, recent data indicate heterogeneity in each group. For example, outcomes may be good in patients whose leukemic blast cells are positive for BCR-ABL fusion and whose disease has a good initial response to prednisone. In 1 study, the estimated 4-year EFS for patients with a good response to prednisone was 55%, whereas that for patients with a poor response was 10% (Schrappe, 1998). Likewise, the estimated 4-year EFS for infants with MLL rearrangements and a good prednisone response was 41%, whereas it was only 9% in those with a poor response to prednisone.

Race: ALL occurs more frequently in Caucasians than in African Americans. The annual incidence of ALL in children and adolescents younger than 15 years in the Caucasian population is 33 per million, compared with 15 per million children and adolescents younger than 15 years in the African American population.

Sex: ALL occurs slightly more frequently in boys than in girls. This difference is most pronounced for T-cell ALL.

Age: The incidence of ALL peaks in children aged 2-5 years.




CLINICAL Section 3 of 11
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History: Children with ALL generally present with signs and symptoms that reflect bone marrow infiltration and extramedullary disease. Because leukemic blasts replace the bone marrow, patients present with signs of bone marrow failure, including anemia, thrombocytopenia, and neutropenia. Clinical manifestations include fatigue and pallor, petechiae and bleeding, and fever. In addition, leukemic spread may manifest as lymphadenopathy and hepatosplenomegaly. Other signs and symptoms of leukemia include weight loss, bone pain, and dyspnea.

Signs or symptoms of CNS involvement, even when it occurs, are rarely observed at the time of the initial diagnosis. The signs and symptoms include headache, nausea and vomiting, lethargy, irritability, nuchal rigidity, papilledema. Cranial nerve involvement, which most frequently involves the seventh, third, fourth, and sixth cranial nerves, may occur. Also, leukemia can involve as intracranial or spinal mass, which causes numerous neurologic symptoms, most of which are due to nerve compression.

Testicular involvement at diagnosis is rare. However, if present, it appears as painless testicular enlargement and is most often unilateral.

Physical: Physical findings in children with ALL reflect bone marrow infiltration and extramedullary disease. Patients present with pallor caused by anemia, petechiae, and bruising secondary to thrombocytopenia. They also have signs of infection because of neutropenia. In addition, leukemic spread may be seen as lymphadenopathy and hepatosplenomegaly.

Careful neurologic examination to look for CNS involvement is important because the treatment for leukemia with CNS involvement is different.

In male patients, testicular examination is necessary to look for testicular involvement of leukemia.

Causes: Although a small percentage of cases are associated with inherited genetic syndromes, the cause of ALL remains largely unknown. DIFFERENTIALS Section 4 of 11
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Acute Myelocytic Leukemia
Anemia, Acute
Anemia, Fanconi
Juvenile Rheumatoid Arthritis
Leukocytosis
Mononucleosis and Epstein-Barr Virus Infection
Neuroblastoma
Non-Hodgkin Lymphoma
Osteomyelitis
Parvovirus B19 Infection
Rhabdomyosarcoma



Other Problems to be Considered:

Aplastic anemia
Idiopathic thrombocytopenic purpura (ITP)

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Acute Myelocytic Leukemia

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Neuroblastoma

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Osteomyelitis

Parvovirus B19 Infection

Rhabdomyosarcoma




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WORKUP Section 5 of 11
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Lab Studies:


Basic laboratory tests
On initial evaluation, obtain a CBC. A hematologist or hematopathologist must evaluate the peripheral smear for the presence and morphology of lymphoblasts. An elevated leukocyte count of >10 X 109/L (>10 X 103/µL) occurs in one half of patients with ALL. The degree of leukocytic elevation (blasts) at diagnosis remains the most important predictor of the patient's prognosis. Neutropenia, anemia, and thrombocytopenia may be observed secondary to inhibition of normal hematopoiesis by leukemic infiltration. Rare cases of ALL may initially manifest with pancytopenia.
Various metabolic abnormalities may include increased serum levels of uric acid, potassium, phosphorus, and calcium, and lactate dehydrogenase (LDH). The degree of abnormality reflects the leukemic cell burden and destruction (lysis). Although not universally performed, coagulation studies can be helpful, including tests of the prothrombin time (PT), activated partial thromboplastin time (aPTT), fibrinogen level, and D-dimer level to assess for disseminated intravascular coagulation; these studies are particularly important in a child who is acutely toxic.
Immunophenotyping
Complete morphologic, immunologic, and genetic examination of the bone marrow is necessary to establish the diagnosis of ALL.
An important advancement in the classification of ALL was the observation that malignant lymphoblasts share many of the features of normal lymphoid progenitors. ALL cells rearrange their immunoglobulin and T-cell receptor (TCR) genes and express antigen receptor molecules in ways that correspond to such processes in normal developing B and T lymphocytes. However, lymphoblasts can also have aberrant gene expression with resultant phenotypes that differ from those of normal lymphocyte progenitors. Nevertheless, ALL can be classified broadly as B- or T-lineage ALL.
The diagnosis of B-cell leukemia, which accounts for only about 3% of ALLs, depends on the detection of surface immunoglobulin on leukemic blasts. Lymphoblasts with this phenotype have a distinctive morphology, with deeply basophilic cytoplasm containing prominent vacuoles. This morphologic pattern is designated L3 in the French-American-British (FAB) system (see Histologic Findings below). Prominent clinical features include extramedullary lymphomatous masses in the abdomen or head and neck and frequent involve the CNS.
Approximately 80% of childhood ALLs involve lymphoblasts with phenotypes that correspond to those of B-cell progenitors. These cases can be identified by their cell-surface expression of 2 or more B-lineage–associated antigens, ie, CD19, CD20, CD24, CD22, CD21, or CD79. Only CD79 is specific for B-lineage ALL. In addition, about one fourth of B-cell precursor cases express cytoplasmic immunoglobulin µ heavy-chain proteins and are designated pre–B-cell ALL. Cases related to B-cell precursors can be subclassified as early pre–B-cell, pre–B-cell, or transitional pre–B-cell cases. Although mature B-cell ALL should be differentiated from B-precursor cases, distinguishing the subtypes of B-precursor ALL is probably not clinically relevant.
T-cell ALL is identified by the expression of T-cell–associated surface antigens, of which cytoplasmic CD3 is specific. T-cell ALL cases can be classified by early, mid, or late thymocytes. Clinical features most closely associated with T-cell ALL are high blood leukocyte counts and CNS involvement. About one half of patients have a mediastinal mass at the time of diagnosis. The prognosis of patients with T-cell ALL has historically been worse than that of patients with B-lineage ALL. However, the outlook for patients with T-cell leukemia appears to improve with intensive chemotherapy.
Cytogenetic and molecular diagnosis
In more than 90% of ALLs, specific genetic alterations can be found in the leukemic blasts. These alterations include changes in chromosome number (ploidy) and structure; about half of all childhood ALLs involve recurrent translocations. Standard cytogenetic analysis is an essential tool in the workup of all patients with leukemia because the karyotype of the leukemic cells has important diagnostic, therapeutic, and prognostic implications. In addition, molecular techniques, including fluorescence in situ hybridization (FISH), reverse transcriptase-polymerase chain reaction (RT-PCR), and Southern blot analysis help improve diagnostic accuracy. Molecular analysis can be used to identify translocations not detected on routine karyotype analysis and to distinguish lesions that appear cytogenetically identical but molecularly different
Clinically important genetic alterations in B-precursor ALL include chromosomal translocations (BCR-ABL, E2A-PBX1, TEL-AML1 gene fusions), a variety of MLL gene rearrangements, and hyperdiploidy. Hyperdiploidy, defined as a DNA index (DI) of >1.16, occurs in about 20% of B-precursor cases and is a favorable prognostic factor. The good outcome of patients with hyperdiploid blasts is probably due to a combination of factors, including increased accumulation of methotrexate (MTX) polyglutamates by leukemic blast cells, sensitivity to antimetabolites, and a propensity for apoptosis. Heterogeneity in the hyperdiploid group is demonstrated by the fact that the outcomes are better in patients with hyperdiploidy and trisomies of chromosomes 4 and 10 than in patients with hyperdiploidy but without both trisomies.
Molecular techniques show that TEL-AML1 fusion, created by t(12;21), is the most common genetic abnormality observed in childhood ALL thus far. This fusion occurs in about 20% of patients and mainly in children aged 3-5 years. This fusion occurs only in B-precursor ALLs, and 50% of these cases express myeloid-associated antigens (CD13, CD33, or both). Many studies have shown that TEL-AML1 fusion is associated with an excellent prognosis, but investigators from 2 studies of relapses question this finding. They reported an incidence of TEL-AML1 of about 20%. Three studies showed a <10% incidence of TEL-AML1 in relapses; this finding is consistent with a favorable prognosis in TEL-AML1–positive ALL.

Therefore, TEL-AML1 fusion appears to be useful in identifying a large subset of patients with B-precursor ALL who have a favorable prognosis. Additional studies are needed to determine whether these patients can be treated successfully with relatively nonintensive, antimetabolite-based therapy.
Assessment of minimal residual disease
Although still experimental, molecular analysis promises to play a role in the diagnosis and treatment of ALL and in monitoring patients' responses to therapy. Studies of minimal residual disease (MRD) may be based on the detection of chimeric transcripts generated by fusion genes, the detection of clonal TCR or immunoglobulin heavy-chain (IgH) gene rearrangements, or the identification of a phenotype specific to the leukemic blasts.
Recent studies have demonstrated that both the presence and level of MRD are correlated with the outcome. A prerequisite to MRD detection in protocol treatment is the ability to apply detection methods in all patients. The author's recent study indicated that immunologic and molecular techniques are equally reliable in detecting clinically significant levels of MRD and that they achieve concordant results. They can be applied in tandem for universal monitoring of MRD in childhood ALL.

Risk classification
With the recognition of distinct prognostic subgroups, contemporary protocols stratify children with B-precursor ALL according to their risk of relapse; low-, standard-, and high-risk groups are generally recognized. Risk classification is partly based on clinical features, the most important of which are age and leukocyte count at the time of diagnosis. Participants at a workshop sponsored by the National Cancer Institute defined the standard-risk group as children aged 1-10 years with an initial leukocyte count of <50 X 109/L; all other patients were considered to have high-risk ALL. When these criteria are used, estimated 4-year EFSs 80% for the standard-risk group and 65% for the high-risk group. However, the EFS for hyperdiploid patients in both risk groups is approximately 89%. This finding suggests that genetic factors may be more accurate than age and leukocyte count as a predictor of outcome.
Further evidence that genetic features of leukemic blasts may be the best factors for risk classification comes from patients with TEL-AM1 expression, who generally have excellent outcomes regardless of their age or leukocyte count. Likewise, infants were previously considered to be a high-risk group. However, now, only infants with MLL rearrangements are assigned to this classification. Outcomes of the 20% of infants without this genetic feature may be similar to those of children older than 1 year. Therefore, a risk-classification scheme based on a combination of clinical features, genetic features, and responses to therapy is used.

According to this scheme, patients with B-lineage ALL and hyperdiploidy or the TEL-AM1 fusion make up the low-risk group, whereas infants with MLL gene rearrangements and patients with BCR-ABL expression make up the high-risk group. All other patients with B-lineage leukemia and all patients with T-cell leukemia are placed into the standard-risk group. However, even specific genetic features are not perfect predictors of outcome. Therefore, additional clinical and biologic information, including rate of cytoreduction, helps refine this classification system and improves the ability to direct treatment.
Imaging Studies:


Chest radiography: Evaluate for a mediastinal mass. In general, no other imaging studies are required. However, if the physical examination reveals enlarged testes, perform ultrasonography to evaluate for testicular infiltration.
Testicular ultrasonography: Perform testicular ultrasonography if the testes are enlarged on physical examination.
Renal ultrasonography: Some clinicians prefer to evaluate for leukemic kidney involvement to assess the risk of tumor lysis syndrome.
Echocardiography and ECG: Obtain an echocardiogram and an ECG before anthracyclines are administered.
Procedures:


Bone marrow aspirate and biopsy: The results confirm the diagnosis of ALL. In addition, special stains (immunohistochemistry), immunophenotyping, cytogenetic analysis, and molecular analysis help in classifying each case.
Lumbar puncture with cytospin morphologic analysis: These tests are performed before systemic chemotherapy is administered to assess for CNS involvement and to administer intrathecal chemotherapy.

Histologic Findings: Although it is not correlated with the immunophenotypic and cytogenetic classification information, the FAB system is generally well accepted and used. According to this system, ALL is classified into 3 groups based on morphology.

L1: Cells are usually small, with scant cytoplasm and inconspicuous nucleoli. L1 accounts for 85% of all cases of childhood ALL.

L2: Cells are larger, than in L1. The cells demonstrate considerable heterogeneity in size, with prominent nucleoli, and abundant cytoplasm. L2 accounts for 14% of all childhood ALLs.

L3: Cells are large and notable for their deep cytoplasmic basophilia. They frequently have prominent cytoplasmic vacuolation and are morphologically identical to Burkitt lymphoma cells. L3 accounts for 1% of childhood ALLs.
TREATMENT Section 6 of 11
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Medical Care: Because leukemia is a systemic disease, therapy is primarily based on chemotherapy. Different forms of ALL require different approaches for optimal results. For example, B-cell ALL does not respond well to the chemotherapy traditionally used for childhood ALL. However, outstanding results, with EFS estimates of nearly 90%, have been obtained with treatments designed for Burkitt lymphoma, which emphasize cyclophosphamide and the rapid rotation of antimetabolites in high dosages. Therefore, B-cell ALL was the first form of ALL to be recognized as a distinct clinical entity on the basis of immunophenotypic and cytogenetic features, and it was the first to be treated by using separate protocols designed specifically for the unique features of this leukemia.

Tumor lysis syndrome
Before and during the initial induction phase of chemotherapy, patients may develop tumor lysis syndrome, which refers to the metabolic derangements caused by the systemic and rapid release of intracellular contents as chemotherapy destroys leukemic blasts. Because some cells can die before therapy, such derangements can occur even before therapy begins.
Primary features of tumor lysis syndrome include hyperuricemia (due to metabolism of purines), hyperphosphatemia, hypocalcemia, and hyperkalemia. The hyperuricemia can lead to crystal formation with tubular obstruction and, possibly, acute renal failure requiring dialysis. Therefore, electrolyte and uric acid levels should be monitored closely throughout initial therapy.
To prevent complications of tumor lysis syndrome, all patients should initially receive intravenous (IV) fluids at twice the maintenance rates, usually without potassium. Sodium bicarbonate is added to the IV fluid to achieve moderate alkalinization of the urine to pH 7.5-8 to enhance the excretion of phosphate and uric acid. Avoid a urine pH higher than this to prevent crystallization of hypoxanthine or calcium phosphate. Administer allopurinol or rasburicase to prevent or correct hyperuricemia.
Phases of therapy

The treatment of childhood ALL, with the exception of B-cell ALL, has 5 components: induction, consolidation, interim maintenance, delayed intensification, and maintenance. The goal of induction is to achieve remission or <5% blasts in the bone marrow. Induction therapy generally consists of 3-4 drugs, which may include a glucocorticoid, a vincristine, an asparaginase, and possibly an anthracycline. This type of therapy induces complete remission in more than 98% of patients.

Consolidation (ie, intensification) therapy is given soon after remission is achieved to further reduce the leukemic cell burden before the emergence of drug resistance and relapse in sanctuary sites (eg, testes, CNS). In this phase of therapy, the drugs given at doses higher than those used during induction, or the patient is given different drugs (eg, high-dose MTX and 6-mercaptopurine (6-MP), epipodophyllotoxins with cytarabine, or multiagent combination therapy). Consolidation therapy, first used successfully to treat patients with high-risk disease, also appears to improve the long-term survival of patients with standard-risk disease. The addition of intensive reinduction therapy (administered soon after remission is achieved) is similarly beneficial for patients in both risk groups.

In interim maintenance, oral medications are administered to maintain remission and allow the bone marrow to recover. This occurs for 4 weeks and is followed by delayed intensification, which is aimed at treating any remaining resistant leukemia cells.

The last phase of treatment is maintenance. This consists of LPs with intrathecal MTX every 3 months, monthly vincristine, daily 6-MP, and weekly MTX.
Duration of therapy:

Whereas B-cell ALL is treated with a 2- to 8-month course of intensive therapy, achieving acceptable cure rates for patients with B-precursor and T-cell ALL requires approximately 2-2.5 years of continuation therapy. Attempts to reduce this time result in high relapse rates after therapy is stopped.

Most contemporary protocols include a continuation phase based on weekly parenterally administered MTX given with daily, orally administered 6-MP interrupted by monthly pulses of vincristine and a glucocorticoid. Although these pulses improve outcomes, they are associated with avascular necrosis of the bone. Patients with high-risk ALL also may benefit from intensified continuation therapy that includes the rotational use of drug pairs.

Improvements in relapse-free survival gained by intensification with anthracyclines or epipodophyllotoxins must be weighed against the late sequelae of these agents, which include cardiotoxicity and treatment-related acute myeloid leukemia.

CNS disease:

Treatment of subclinical CNS leukemia is an essential component of ALL therapy.

Although cranial irradiation effectively prevents overt CNS relapse, concern about subsequent neurotoxicity and brain tumors has led many investigators to replace irradiation with intensive intrathecal and systemic chemotherapy for most patients. This strategy has produced excellent results, with CNS relapse rates of <2% in some studies.

Whether cranial irradiation is necessary for patients with very high-risk ALL (patients with BCR-ABL or MLL gene rearrangements) is unclear.

High-risk patients:

Optimal treatment for patients with very high-risk ALL has not been found.

Many institutions treat these patients with allogeneic stem-cell transplantation (SCT) soon after first remission is achieved. For patients without a matched family donor, transplantation of marrow from an unrelated donor is a reasonable treatment option. Results of SCT, often reported from single institutions, have been inconsistent and sometimes disappointing. Large, multi-institutional, controlled trials are clearly needed to determine the effectiveness of this therapy for patients without a matched donor.

Treatment of relapse: In general, relapsed ALL cells acquire resistance to exposed chemotherapy drugs. Therefore, treatment of relapse is intensive and often including SCT and new drugs. However, the outcome of relapse is poor.

Molecular targeted therapy

The principle that a drug targeted at the underlying molecular defect which is unique to certain leukemias can have potent and specific antileukemic activity while producing minimal toxicity to normal cells.

This approach has been evolving in the treatment of leukemia.

The best example is imatinib mesylate, a selective BCR-ABL tyrosine kinase inhibitor. Imatinib mesylate has demonstrated significant anti-leukemic activity and is now a standard frontline treatment for Ph-positive chronic myeloid leukemia (CML). Imatinib mesylate is also effective in Ph-positive ALL, and combination regimens with imatinib mesylate and conventional chemotherapy or SCT have been evaluated in trials.

Impact of genetic studies and future challenges:

More than 80% of children with ALL now can be cured. However, the cause of treatment failure in the remaining 20% of patients is largely unknown.

Because of the diverse nature of the disease, use of risk-directed therapy for all patients on the basis of molecular and pharmacogenetic characterization of the leukemic cells at the time of diagnosis is favored.

Microarray, improved multiparameter flow-cytometric analysis, quantitative RT-PCR, genomics, proteomics and sophisticated bioinformatics, and computing technologies hold real promise for providing important clues to the mechanisms behind leukemogenesis and response and resistance to current therapies.

Future goals include the use of these technologies to identify additional biologic subsets of ALL that require specifically targeted therapies.
Surgical Care: Surgical care is generally not required in the treatment of ALL, except for the placement of a central venous catheter. Such catheters are used for administering chemotherapy, blood products, and antibiotics, and for drawing blood samples.

Consultations: A number of consultations should be obtained depending on the clinical circumstances of patients with newly diagnosed ALL.

Pediatric oncologist: Refer all patients to a subspecialist to direct their care.
Pediatric surgeon: Patients require placement of a central venous catheter.
Psychosocial team: Involve psychologists and social workers in the care of patients with ALL to aid them and their families in navigating all of the difficult issues surrounding their care.
Radiation oncologist: Depending on their risk group, some patients require craniospinal radiation as part of the treatment plan.
Other subspecialists: Consultations with other specialists (eg, infectious disease specialist, nephrologist) may be appropriate depending on the clinical circumstances.
Diet: Because of the use of MTX, avoid folate supplementation.
MEDICATION Section 7 of 11
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Drugs commonly used during remission induction therapy include dexamethasone or prednisone, vincristine, asparaginase, and daunorubicin. Consolidation therapy often includes MTX and 6-MP. Drugs used for intensification or continuation include cytarabine, cyclophosphamide, etoposide, dexamethasone, asparaginase, doxorubicin, MTX, 6-MP, and vincristine. Intrathecal chemotherapy includes MTX, hydrocortisone, and cytarabine.


Drug Category: Antineoplastics agents -- Cancer chemotherapy is based on an understanding of tumor cell growth and how drugs affect this growth. After cells divide, they enter a period of growth (ie, phase G1), followed by DNA synthesis (ie, phase S). The next phase is a premitotic phase (ie, G2), then finally a mitotic cell division (ie, phase M).

Cell-division rates vary for different tumors. Most common cancers grow slowly compared with normal tissues, and the rate may be decreased in large tumors. This difference allows normal cells to recover from chemotherapy more quickly than malignant ones and is the rationale behind current cyclic dosage schedules.

Antineoplastic agents interfere with cell reproduction. Some agents are specific to phases of the cell cycle, whereas others (eg, alkylating agents, anthracyclines, cisplatin) are not. Cellular apoptosis (ie, programmed cell death) is another potential mechanism of many antineoplastic agents.Drug Name
Prednisone (Deltasone) -- Corticosteroid. Important chemotherapeutic agent in treatment of ALL. Used in induction and reinduction therapy. Also given as intermittent pulses during continuation therapy.
Adult Dose 20-25 mg PO tid
Pediatric Dose 40 mg/m2/d PO divided tid
Contraindications Documented hypersensitivity; serious infections (excluding meningitis and septic shock) and fungal infections; varicella infections
Interactions May potentiate thrombogenic effects of asparaginase; barbiturates, phenytoin; rifampin may decrease effectiveness
Pregnancy B - Usually safe but benefits must outweigh the risks.
Precautions Gradual tapering of dose required after prolonged treatment (ie, >2 wk); toxicity includes fluid retention, hypertension, increased appetite, transient diabetes, acne, striae, personality changes, peptic ulcer, immunosuppression, osteoporosis, growth retardation; caution in diabetes, fungal infections, and osteonecrosis
Drug Name
Dexamethasone (Decadron, Dexone) -- Corticosteroid. Important chemotherapeutic agent in treatment of ALL. Used in induction and reinduction therapy. Also given as intermittent pulses during continuation therapy.
Adult Dose 6-8 mg/m2/d PO divided tid
Pediatric Dose Administer as in adults
Contraindications Documented hypersensitivity; serious infections (excluding meningitis and septic shock) and fungal infections; varicella infections
Interactions May potentiate thrombogenic effects of asparaginase; barbiturates, phenytoin; rifampin may decrease effectiveness
Pregnancy C - Safety for use during pregnancy has not been established.
Precautions Gradually taper after prolonged use; adverse effects include gastritis, hypertension, hyperglycemia, salt and water retention, personality changes, growth retardation, osteoporosis; caution in diabetes and osteonecrosis
Drug Name
Vincristine (Oncovin, Vincasar) -- Chemotherapeutic agent derived from periwinkle plant. Inhibits microtubule formation in mitotic spindle, causing metaphase arrest.
Adult Dose Induction therapy: 2 mg IV qwk
Continuation therapy: 2 mg IV every mo
Pediatric Dose 1.5 mg/m2 IV; not to exceed 2 mg/dose
Contraindications Documented hypersensitivity; demyelinating form of Charcot-Marie-Tooth syndrome; intrathecal administration
Interactions Acute pulmonary reaction may occur with concurrent mitomycin-C; asparaginase, cytochrome P450 (CYP) 3A4 inhibitors (eg, itraconazole, quinupristin/dalfopristin, sertraline, ritonavir), granulocyte-macrophage colony-stimulating factor (GM-CSF, eg, sargramostim, filgrastim), or nifedipine increase toxicity; CYP3A4 inducers (eg, carbamazepine, phenytoin, phenobarbital, rifampin) may decrease effects; zidovudine increases risk of bone marrow suppression
Pregnancy D - Unsafe in pregnancy
Precautions Peripheral neuropathy manifested by constipation, ileus, ptosis, vocal cord paralysis, jaw pain, abdominal pain, loss of deep tendon reflexes; reduce dosage with severe peripheral neuropathy; bone marrow depression; local ulceration with extravasation, syndrome of inappropriate antidiuretic hormone secretion (SIADH)
Drug Name
Asparaginase (Elspar, Kidrolase) -- Extracts of Escherichia coli or Erwinia L-asparaginase impair asparagine synthesis. Lethal to cells that cannot synthesize essential amino acid asparagine.
Adult Dose Induction therapy: 6000-25,000 U/m2 IM 3 times/wk
Continuation therapy: Administer qwk
Pediatric Dose Administer as in adults
Contraindications Documented hypersensitivity; history of pancreatitis
Interactions Possible inhibition of MTX effect; possible increased toxicity with vincristine or prednisone
Pregnancy C - Safety for use during pregnancy has not been established.
Precautions Hypersensitivity reactions with local rash, hives, anaphylaxis; bone marrow depression, hyperglycemia, hepatotoxicity, and bleeding may occur
Drug Name
Daunorubicin (Cerubidine) -- Anthracycline that intercalates with DNA and interferes with DNA synthesis.
Adult Dose 25 mg/m2 IV qwk during induction therapy
Pediatric Dose Administer as in adults
Contraindications Documented hypersensitivity; congestive heart failure, arrhythmias, or cardiopathy
Interactions Coadministration of trastuzumab increases cardiotoxic effects
Pregnancy D - Unsafe in pregnancy
Precautions Myelosuppression and thrombocytopenia; may cause cardiac arrhythmias immediately after administration and cardiomyopathy after long-term use; nausea, vomiting, stomatitis, and alopecia; extravasation may occur, resulting in severe tissue necrosis; caution in impaired hepatic, renal, or biliary function
Drug Name
MTX (Folex PFS) -- Folate analog that competitively inhibits dihydrofolate reductase, inhibiting DNA, RNA, and protein synthesis.
Adult Dose 20-8000 mg/m2 PO/IV/IM qwk to every mo, depending on protocol
Pediatric Dose Administer as in adults
Contraindications Documented hypersensitivity; alcoholism, hepatic insufficiency, documented immunodeficiency syndromes, preexisting blood dyscrasias (eg, bone marrow hypoplasia, leukopenia, thrombocytopenia, significant anemia)
Interactions Concurrent PO aminoglycosides may decrease absorption and blood levels; charcoal lowers levels; coadministration with etretinate may increase hepatotoxicity; folic acid or its derivatives contained in some vitamins may decrease response; coadministration with nonsteroidal anti-inflammatory drugs (NSAIDs) may be fatal; indomethacin and phenylbutazone can increase plasma levels; may decrease phenytoin serum levels; probenecid, salicylates, procarbazine, and sulfonamides, including trimethoprim-sulfamethoxazole (TMP-SMZ), may increase effects and toxicity; may increase plasma levels of thiopurines
Pregnancy X - Contraindicated in pregnancy
Precautions Hematologic, renal, GI, pulmonary, and neurologic systems; discontinue if blood counts substantially decrease; aspirin, NSAIDs, or low-dose steroids may be administered concomitantly; increased toxicity with NSAIDs, including salicylates, not tested
Drug Name
6-MP (Purinethol) -- Synthetic purine analog that kills cells by incorporating into DNA as false base.
Adult Dose 50-75 mg/m2/dose PO qd
Pediatric Dose Administer as in adults
Contraindications Documented hypersensitivity
Interactions Increased toxicity with allopurinol; increased hepatic toxicity when combined with doxorubicin
Pregnancy D - Unsafe in pregnancy
Precautions Renal or hepatic impairment; high risk of pancreatitis; monitor for myelosuppression
Drug Name
Cytarabine (Cytosar-U) -- Synthetic analog of nucleoside deoxycytidine. Undergoes phosphorylation to arabinofuranosyl-cytarabine-triphosphate (ara-CTP), competitive inhibitor of DNA polymerase.
Adult Dose Induction therapy: 300-3000 mg/m2 IV qid
Continuation therapy: Pediatric Dose Administer as in adults
Contraindications Documented hypersensitivity; cerebellar toxicity
Interactions Decreased effects of gentamicin and flucytosine; increased toxicity with other alkylating agents and radiation
Pregnancy D - Unsafe in pregnancy
Precautions Severe leukopenia and thrombocytopenia; immunosuppression, nausea, vomiting, anorexia, stomatitis, GI ulceration, fever, alopecia, and rash; cerebellar toxicity and ataxia may develop
Drug Name
Etoposide (Toposar, VePesid) -- Inhibits topoisomerase II and breaks DNA strands, causing cell proliferation to arrest in late S or early G2 portion of cell cycle.
Adult Dose 300 mg/m2 IV, frequency depends on protocol; often not used
Pediatric Dose Administer as in adults
Contraindications Documented hypersensitivity; IT administration may cause death
Interactions May prolong effects of warfarin and increase clearance of MTX; with cyclosporine, has additive effects on cytotoxicity of tumor cells
Pregnancy D - Unsafe in pregnancy
Precautions Myelosuppression; secondary acute myeloid leukemia
Drug Name
Cyclophosphamide (Cytoxan) -- Chemically related to nitrogen mustards. As alkylating agent, mechanism of action of active metabolites may involve cross-linking of DNA, which may interfere with growth of normal and neoplastic cells.
Adult Dose Induction therapy: 300-1000 mg/m2 IV once
Continuation therapy: Pediatric Dose Administer as in adults
Contraindications Documented hypersensitivity; severely depressed bone marrow function
Interactions Possibly increased risk of bleeding or infection and enhanced myelosuppressive effects with coadministration of allopurinol; may potentiate doxorubicin-induced cardiotoxicity; may reduce digoxin serum levels and antimicrobial effects of quinolones; chloramphenicol may increase half-life while decreasing metabolite concentrations; may increase effect of anticoagulants; coadministration with high doses of phenobarbital may increase rate of metabolism and leukopenic activity of cyclophosphamide; thiazide diuretics may prolong cyclophosphamide-induced leukopenia and neuromuscular blockade by inhibiting cholinesterase activity.
Pregnancy D - Unsafe in pregnancy
Precautions Alopecia, nausea, vomiting, stomatitis, diarrhea, myelosuppression, immunosuppression, hemorrhagic cystitis, SIADH; may cause sterility in male patients
-- Drug Name
Nelarabine (Arranon)- Prodrug of deoxyguanosine analog 9-beta-D-arabinofuranosylguanine (ara-G). Converted to active 5'-triphosphate, arabinofuranosyl-guanine-5'-triphosphate (ara-GTP), T-cell–selective nucleoside analog. Leukemic blast cells accumulate ara-GTP. This allows for incorporation into DNA, leading to inhibition of DNA synthesis and cell death.
Approved by US Food and Drug Administration [FDA] as orphan drug to treat T-cell lymphoblastic lymphoma (type of non-Hodgkin lymphoma [NHL]) that does not respond or that relapsing with at least 2 chemotherapy regimens.
Adult Dose 1500 mg/m2 IV (infuse over 2 h) on days 1, 3, and 5; repeat q21d
Pediatric Dose 650 mg/m2 IV (infuse over 1 h) qd for 5 consecutive days; repeat q21d
Contraindications Documented hypersensitivity
Interactions None reported
Pregnancy D - Fetal risk shown; may use if benefits outweigh risk to fetus.

Precautions Common adverse effects include hematologic toxicity (eg, leukopenia, thrombocytopenia, anemia, neutropenia), hypokalemia, hypoalbuminemia, hyperbilirubinemia, fatigue, nausea, vomiting, and diarrhea; severe neurologic events reported and include extreme somnolence, convulsions, demyelination, ascending peripheral neuropathies similar to Guillain-Barré syndrome, and peripheral neuropathy ranging from numbness and paresthesia to motor weakness and paralysis; do not dilute before administration; preventive measures for hyperuricemia of tumor lysis syndrome (eg, hydration, urine alkalinization, allopurinol prophylaxis) must be taken
Drug Name
Clofarabine (Clolar) -- Purine nucleoside antimetabolite that inhibits DNA synthesis. Pools of cellular deoxynucleotide triphosphate decreased by inhibiting ribonucleotide reductase and terminating DNA chain elongation and repair. Also disrupts mitochondrial membrane integrity. Indicated for relapsed or refractory ALL in pediatric patients.
Adult Dose >21 years: Not established
Pediatric Dose <1 year: Not established
1-21 years: 52 mg/m2 IV infused over 2 h qd for 5 consecutive days; repeat cycle after recovery or return to baseline organ function (about q2-6wk)
Contraindications None known
Interactions Avoid coadministration with drugs toxic to kidneys or liver (eg, aminoglycosides, amphotericin B, loop diuretics, inhaled anesthetics, high doses of acetaminophen)
Pregnancy D - Unsafe in pregnancy
Precautions Because of rapid reduction in leukemia cells after treatment, may cause tumor lysis syndrome and cytokine release (eg, tachypnea, tachycardia, hypotension, pulmonary edema) that may develop into systemic inflammatory response syndrome or capillary leak syndrome and organ dysfunction; may cause bone marrow depression and risk of severe opportunistic infections; may cause vomiting, diarrhea, and subsequent dehydration
Drug Category: Prophylactic antimicrobials -- These drugs are given to prevent infection in patients receiving chemotherapy.Drug Name
SMZ and TMP (Cotrim, Septra, Bactrim) -- Inhibits bacterial growth by inhibiting synthesis of dihydrofolic acid. All immunocompromised patients should be treated with cotrimoxazole to prevent Pneumocystis carinii pneumonia (PCP).
Adult Dose 2 tabs PO bid 3 d/wk; alternatively 1 double-strength tab bid 3 d/wk
Pediatric Dose 5-10 mg/kg/d (based on TMP component) PO divided q12h 3 times/wk
Contraindications Documented hypersensitivity; megaloblastic anemia due to folate deficiency
Interactions May increase PT when used with warfarin (perform coagulation tests and adjust dose accordingly); most other interactions minor in severity when dosed 3 times/wk
Pregnancy C - Safety for use during pregnancy has not been established.
Precautions Discontinue at first appearance of rash or sign of adverse reaction; caution in folate deficiency; hemolysis may occur in individuals with glucose-6-phosphate dehydrogenase (G-6-PD) deficiency; patients with AIDS may not tolerate or respond to TMP-SMZ
Drug Name
Nystatin (Nilstat) -- Used to prevent fungal infections in mucositis. Fungicidal and fungistatic antibiotic from Streptomyces noursei; effective against various yeasts and yeastlike fungi. Changes permeability of fungal cell membrane after binding to cell membrane sterols, causing cellular contents to leak.
Treatment should continue until 48 h after symptoms disappear. Not substantially absorbed from GI tract.
Adult Dose 10 mL PO swish and swallow qid
Pediatric Dose 5 mL PO swish and swallow qid
Contraindications Documented hypersensitivity
Interactions None reported
Pregnancy C - Safety for use during pregnancy has not been established.
Precautions Not for treatment of systemic fungal infections
Drug Name
Clotrimazole troches (Mycelex) -- May be used instead of nystatin to prevent fungal infections. Broad-spectrum antifungal agent that inhibits yeast growth by altering cell membrane permeability, causing death of fungal cells.
Adult Dose 1 troche dissolved PO qid
Pediatric Dose Administer as in adults
Contraindications Documented hypersensitivity
Interactions None reported
Pregnancy B - Usually safe but benefits must outweigh the risks.
Precautions Not for treatment of systemic fungal infections; avoid contact with eyes; if irritation or sensitivity develops, discontinue and start appropriate therapy
Drug Name
Itraconazole (Sporanox) -- Used to prevent fungal infections in high-risk patients. Fungistatic activity. Synthetic triazole antifungal agent that slows fungal cell growth by inhibiting CYP-dependent synthesis of ergosterol, vital component of fungal cell membranes. Bioavailability greater for PO solution than for cap.
Adult Dose 200-400 mg/d PO
Pediatric Dose 10 mg/kg/d PO
Contraindications Documented hypersensitivity; coadministration with cisapride may cause adverse cardiovascular effects (possibly death)
Interactions Inhibits CYP3A4; antacids may reduce absorption; edema may occur with coadministration of calcium channel blockers (eg, amlodipine, nifedipine); hypoglycemia may occur with sulfonylureas; may increase tacrolimus and cyclosporine plasma concentrations when high doses are used; rhabdomyolysis may occur with coadministration of 3-hydroxy-3-methylgluatryl coenzyme A reductase (HMG-CoA) reductase inhibitors (lovastatin or simvastatin); coadministration with cisapride can cause cardiac rhythm abnormalities and death; may increase digoxin levels; coadministration may increase plasma levels of CYP3A4 substrates (eg, midazolam, triazolam, cyclosporine); phenytoin and rifampin may reduce levels (may alter phenytoin metabolism)
Pregnancy C - Safety for use during pregnancy has not been established.
Precautions Caution in hepatic insufficiencies
FOLLOW-UP Section 8 of 11
Author Information Introduction Clinical Differentials Workup Treatment Medication Follow-up Miscellaneous Pictures Bibliography





Further Inpatient Care:


Frequent hospitalizations may be required to deal with complications of therapy, including the need for blood transfusions or antibiotics.
Immediately admit any patient who is neutropenic and who develops chills or fever to administer IV broad-spectrum antibiotics.
Further Outpatient Care:


Frequent clinic visits are required to administer outpatient chemotherapy, to monitor blood counts, and to evaluate new symptoms.
In/Out Patient Meds:


Pneumocystis prophylaxis: All patients should be on TMP-SMZ to prevent PCP.
Fungal prophylaxis: Patients should be given oral nystatin or clotrimazole (Mycelex) troches to prevent candidiasis. Patients with a high risk of relapse should also be taking daily itraconazole.
Mouth care: Patients should swish and spit with an antimicrobial, such as chlorhexidine (Peridex) or antibacterial enzymatic mouthwash (Biotene), 4 times a day.
Transfer:


Initially transfer patients to a facility in which they can be in the care of a pediatric oncologist, preferably a center that participates in multi-institutional clinical trials.
Deterrence/Prevention:


Because the cause of ALL is unknown, no method of prevention is known.
Complications:


Complications of leukemia and its therapy include the following:
Tumor lysis syndrome
Renal failure
Sepsis
Bleeding
Thrombosis
Typhlitis
Neuropathy
Encephalopathy
Seizures

Secondary malignancy

Short stature (if craniospinal radiation)

Growth hormone deficiency

Cognitive defects
Prognosis:


Overall, the cure rate for childhood ALL is over 80%.
However, the prognosis depends on the clinical and laboratory features described above.
In general, the prognosis is best for children aged 1-10 years.
Adolescents have intermediate outcomes.
Infants younger than 1 year have a poor outcome, with cure rates of about 30%.
Patient Education:


Ensure that the patient's parents and guardians have a reasonable understanding of the expected adverse effects of each medication.
In addition, parents and guardians must be able to recognize signs and symptoms that require medical attention, such as signs and symptoms of anemia, thrombocytopenia, and especially infection.
Parents must know how to quickly access medical help from the oncology team.
For excellent patient education resources, visit eMedicine's Cancer and Tumors Center. Also, see eMedicine's patient education article Leukemia.