2006-07-06 06:08:11 · 6 answers · asked by Anonymous in Science & Mathematics ➔ Biology
To get the DNA into the bacteria, we have to poke holes in them with the chemical calcium chloride (CaCl2). CaCl2 will dissociate into Ca2+ and 2 Cl-, and the positive charge of the Ca2+ cancels the negative charge of the DNA, allowing it to cross the cell wall and cell membrane. The holes poked to allow the DNA in leaves the bacteria leaky. If we don't keep them on ice, they'll 'bleed' to death.
2006-07-11 04:59:33 · answer #1 · answered by Anonymous · 1⤊ 1⤋
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2016-03-18 04:09:47 · answer #2 · answered by Anonymous · 0⤊ 1⤋
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RE:
why we use calcium chloride(CaCl2) in transformation of bacteria?
2015-08-25 10:58:15 · answer #3 · answered by Idell 1 · 0⤊ 0⤋
After the cell wall of the bacteria is disolved by the SDS, the Calcium chloride attaches to the pores of the bacteria and opens them up. This gives the desired plasmid a chance to enter into the cell.
2006-07-06 08:19:27 · answer #4 · answered by kameron_richardson 2 · 1⤊ 0⤋
The bacteria used widely is E. coli which is a gram negative bacterium . It contains LPS on its outer surface . now whenever a treatment of cacl2 is given ca2+ and cl- dissociates here calcium nullifies negative charge of DNA as well as LPS and thereby integrity of cell membrane loosens and pores are created .
2014-11-16 14:35:24 · answer #5 · answered by Srishti 1 · 0⤊ 0⤋
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2016-04-07 00:57:30 · answer #6 · answered by Anonymous · 0⤊ 0⤋
It stabilizes the ions at the cell surface and creates pores in the membrane that allow the DNA to go through
2006-07-06 06:19:11 · answer #7 · answered by TonySti 2 · 0⤊ 0⤋
Its a good dessicant
2006-07-06 06:12:23 · answer #8 · answered by ag_iitkgp 7 · 0⤊ 0⤋
Why it uses the concentration at 50mM CaCl2 ??
2015-01-31 15:00:51 · answer #9 · answered by Supinya Th 1 · 0⤊ 0⤋