what is the influence of high degree of cholesterol in liposome composition (1/3) on sonication process?

Question

I've tried to prepare SUV as a part of DRV preparation (using sucrose), but I don't get a translucent solution (hazy) after 40 minutes (netto) of bath sonication. Might it be because the high degree of cholesterol in liposome composition? Am I supposed to get the translucent solution with cholesterol? What are the conditions for getting translucent solution with bath sonicator or tip(prop) conicator.

Thanks in advance

Answer 1

No, try setting the temp. correctly.