What will happen if bacteria is incubated for more than 48 hours?

Question

I am doing a science fair experiment involving bacteria growth. I have the incubator set to right around 100 degrees Farenheight. What, [if anything] will happen if it is incubated for more than 48 hours? Will it grow more or hamper it in someway?

Answer 1

I doubt the bacteria will run out of nutrients in 48 hours, but if you're planning to analyze the plates by counting the number of colonies that grow, there's a good chance that several small colonies can grow together so they may look like a single colony, so any counts would be less than the actual number of bacteria that were present.

Also, some types of bacteria may inhibit the growth of others, if not kill them, if they come into contact with one another.

Answer 2

Bacteria continue growing until they use up the nutrients or produce enough toxins to stop their growth. If you have nice colonies formed after 24 hours, they grow larger in 48, and may overgrow the plate in 96.

Answer 3

first the higher than normal body temperature might slightly impede growth but in actuality most of the growth will occur within the first 24 to 36 hours, some growth will continue after that but mostly the cells slow down due to a lack of space and loss of nutrients. if you are staining for gram stain purposes you will get false positives if taken after 24 hours. only fresh bacterial growth should be used (first 24 hours)

Answer 4

lysozyme ia a protease which will cut back the derived proteins, so no longer suited. detergent or surfactant additionally reason structural disturbances and denaturation of proteins so no longer suited. anitbiotics, besides being costly are no longer undemanding to split from the broth in later stages, so no longer suited. transpeptidase in fact flow hyperlinks and aids in forming cellular partitions so can't be used for keeping apart the two communities. EDTA is as a result the main favored technique for keeping apart the two communities on lab scale.

Answer 5

The bacteria will continue to grow at their maximum rate until they run out of space or nutrients.
Imagine a field full of rabbits. They will continue to breed and multiply until they eat all the grass, then their rate of reproduction will fall away as they starve to death, because the greedy little gits have eaten all the grass... :-)

PS, 'higher than normal body temp' would have absolutely zero effect on any organism, unless its optimum temperature was 'normal body temp' If this particular bacterium's optimum temp was 100º F then it would be quite at home.

PPS. Any chance of you guys dragging yourselves into the 21st century and ditching this archaic 'fahrenheit' nonsense?

Answer 6

grow more