does it matter which of the DNA chains (the strands that were separated) is used for transcription by RNA?

Question

For Example, the DNA sequence would be:
G-C
A-T
T-A
C-G
A-T

If the RNA pairs on the first side then it would be
G-C
A-U
T-A
C-G
A-U

the other side would be:
C-G
T-A
A-U
G-C
T-A

so does it matter???

Answer 1

Actually the other side would be:
T-A
G-C
A-U
T-A
C-G

So the RNAs produced would be either:

5'-UGAUC-3'
or
5'-ACUAG-3'

These are totally different sequences, so yes, it matters.

Answer 2

One strand of DNA, the template strand (or non-coding strand), is used as a template for RNA synthesis. As transcription proceeds, RNA polymerase traverses the template strand and uses base pairing complementarity with the DNA template to create an RNA copy. Although RNA polymerase traverses the template strand from 3' → 5', the coding (non-template) strand is usually used as the reference point, so transcription is said to go from 5' → 3'. This produces an RNA molecule from 5' → 3', an exact copy of the coding strand (except that thymines are replaced with uracils, and the nucleotides are composed of a ribose (5-carbon) sugar where DNA has deoxyribose (one less Oxygen atom) in it's sugar-phosphate backbone).

Answer 3

Dna Chains

Answer 4

It absolutely does matter! mRNA is transcribed from DNA by an enzyme called RNA polymerase. RNA polymerase looks for certain nucleotide sequences as a clue for where to being transcribing. These special sequences are called promoter regions. In eukaryotes, promoter regions often contain a T-A-T-A-A-A nucleotide sequence (called a TATA box). In a gene, only one strand of DNA has this region, so only one strand serves as a template for transcription. The other strand merely serves to protect the template strand when transcription is not occurring, and of course it serves as a template strand itself during replication.

So only one strand in a gene is transcribed, but is it always the same strand? As it turns out, the answer is no. The strand that serves as a template for transcription in some genes becomes a non-template strand for other genes. But in no cases are both strands transcribed for the same stretch of DNA.

Good question, by the way!

Answer 5

Yes, a specific gene is only on one strand of DNA. There are different genes on both strands of the DNA.

Answer 6

no
no matter which strand is used they will still be identical
because that is what protein synthesis is