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recently, a scientist in the midwestern United States claimed to have found the largest living organism, a mushroom-bearing fungus in Michigan. How might the scientist know that the mycelia that consitute the body of the fungus, mostly growing below ground, is in fact a single organism?

answer this and be specific because i am stuck for 10 points

2007-12-02 05:27:09 · 5 answers · asked by star baller 360 5 in Science & Mathematics Biology

5 answers

The mycelia all grow out from one fruiting body.
researchers collected samples of fungus from 9.65 square km of discontinuous dead patches in the Oregon forest and grew them together in laboratory Petri dishes.
The results confirmed the identical genetic make-up of all the samples.
It's one organism that began as a microscopic spore and then grew vegetatively, like a plant.

2007-12-02 05:41:03 · answer #1 · answered by Ethereal 4 · 0 0

The study of Smith et al. (1992) was performed by collecting vegetative mycelium of Armillaria by "baiting" with small pieces of poplar wood, actually popsicle stick-like tongue depressors. Since Armillaria is a wood decay fungus, the mycelium quickly colonized the tongue depressors. The labeled inoculum stick could then be easily collected. Additional subcultures, including tissues and single spore isolates, were made directly from fruiting bodies that appeared in the fall or from the black rhizomorphs and mycelial fans that are always present in the soil or on the wood, especially under the bark. The laborious process of analysis began, first with checking the mating type loci by mating on media in Petri dishes. Molecular techniques were then employed, first looking at mitochondrial DNA (mDNA) restriction patterns. These were both good markers for the study because mating type loci and mDNA restriction patterns are both highly variable within Armillaria species. Once these were determined, RAPD (Random Amplified Polymorphic DNA) and RFLP (Restriction Fragment Length Polymorphisms) markers were employed to check for additional heterozygous loci in the nuclear genome. With these several types of data, they could begin to draw maps of the area to determine the limits of each individual. One of these clones turned out to be quite large, covering 15 hectares (37 acres). Within this area, all the vegetative isolates had the same mating type , the same mDNA restriction pattern, and had the same eleven RAPD products and five RFLP-based markers, each marking a heterozygous locus. These data indicated that this 15 hectare clone is a single organism.

2007-12-02 05:44:42 · answer #2 · answered by Anonymous · 0 1

dna

2007-12-02 05:31:09 · answer #3 · answered by doug4jets 7 · 1 3

Say what?

2007-12-02 05:34:27 · answer #4 · answered by Anonymous · 0 3

well um idk thts a tricky one sory i cnt help

2007-12-02 05:43:47 · answer #5 · answered by Beautiful<3Disaster 2 · 0 2

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