English Deutsch Français Italiano Español Português 繁體中文 Bahasa Indonesia Tiếng Việt ภาษาไทย
All categories

1 answers

There's more than one possible reason..

But basically one or both of the primers are annealling to more than one spot on a template or templates.

This could be due to a flaw in the design of one or both of the primers, causing them to anneal to a related but different sequence, or it could be due to a repetive sequence in the desired amplification target.

2007-11-27 18:27:07 · answer #1 · answered by BP 7 · 0 0

fedest.com, questions and answers