I recently did an experiment and It was affecting the temperature on enzyme activity. one would expect that the graph would increase and then hit a point of denaturation and then decrease. This happened excpet for at the point of 40 C it decreased slightly and then increased again. What could be the cause of this?
2007-11-20 14:42:53 · 2 answers · asked by Anonymous in Science & Mathematics ➔ Biology
Well I could think of one explanation is that there are another enzyme present which has highest activity at 40 C and the action of the second enzyme is to inhibit your first enzyme. Hence at 40 C your first enzyme activity decreases slightly. And generally with increase in temperature, your enzyme activity will increase given the temperature is not too high to cause denaturation.
2007-11-20 15:41:15 · answer #1 · answered by Anonymous · 0⤊ 0⤋
Good question - generally, if we want to denature proteins, to run out on a gel for instance, we whack them at anything from 70 to 90C. For what it's worth, there are several enzymes we commonly use that hit their peak activity at somewhere around 50-55C, so you may still be a bit low. I'd suppose it would depend on the enzyme, too.
2007-11-20 14:50:02 · answer #2 · answered by John R 7 · 0⤊ 0⤋