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1. effectiveness of staining procedure (following correct procedure with high quality, fresh reagents) as well as appropriate type of stain (wright's geimsa for blood smears, gram stain for bacteria, etc.)
2. thickness of preparation.
3. resolution of scope
4. effectiveness of light source for the type of cell you are looking for (phase contrast, darkfield, regular light microscope, fluorescent scope--for use with fluorescent dyes)
5.correct adjustment of microscope (diaphragm, condensor, focusing)

2007-11-17 13:11:46 · answer #1 · answered by bad guppy 5 · 0 0

resolution
contrast

2007-11-17 20:43:56 · answer #2 · answered by bronte heights 6 · 0 0

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