ethidium bromide sensivity?

Question

It is disprortionately the high sensitivity of ethdium at low DNA concs that is probably causing the lack of valleys between peaks. Putting it another way, its lack of linearity at high DNA concs. This is well known as a general property of fluorescence.
is that true and where i can find out more about this?

Answer 1

This sounds to me like self-quenching, where electronic energy is transferred from one dye molecule to another, being converted to vibrational energy in a series of steps, rather than being emitted as light.

Look up "self-quenching" in any textbook that explains fluorescence.