Trying to make 50x TAE but added 1/2 amount of tris base, how do i fix?

Question

Here's the recipe for 50X TAE buffer
first add about 900ml dH2O
242 g Tris Base
57.1 ml acetic acid
18.6 g EDTA
Adjust pH to 8.1
Add dH2O to 1000ml

i only added 121 g of Tris but i already have 1L total. how do i fix this? thanks.

Answer 1

2 ways:
1) make 2 or 1.5 L and don't mess up again

2) take some time using algebra and calculate the ratio's and add in what else you need to equalize it

Answer 2

Most of us have done that sort of thing at one time or other. I would just add enough T, A and E for 1.5L and adjust the volume to that. Incidentally, that's why I usually start with about half the volume of water (500 mL in this case) to make these solutions - that way, when I realize I've fouled up, I can correct it with less trouble...

Answer 3

Just add the rest of the Tris, it won't make that much of a difference in total volume :)

Or you could make 25X TAE instead by adding ddw to 2000ml :p