A chemist started to carry out column chromatography on Friday afternoon, reached the point at which the two compounds being separated were about three-fourths of the way down the column, and then returned on Monday to find that the compound came off the column as a mixture. Sepculate the reason for this. The column had not run dry over the weekend
2007-10-25 17:23:40 · 2 answers · asked by *QP* 1 in Science & Mathematics ➔ Chemistry
It sounds like the mobile phase may not have been the ideal solvent for separating the compounds. Sometimes, you can alter the temperature of the column or change the flow rate of the mobile phase through the column to get better separation. If that doesn't work, try a new mobile phase.
2007-10-25 17:36:00 · answer #1 · answered by Anonymous · 0⤊ 0⤋
Because he left it, the first compound came out then the second one went into the same vessel as nobody there to change vials.
Or the solvent being used acheived no separation because it carried both materials down the column at the same speed (try a different solvent or solvent mixture)
2007-10-26 00:31:56 · answer #2 · answered by Flying Dragon 7 · 0⤊ 0⤋