agglutination experiment, i'm really stuck..please help?

Question

background:
Rabbits have been artificially immunized with sheep red blood cells in order to stimulate the production of agglutination and lysis. In this experiment the agglutination of the rabbit serum is measured. So I need to determine the agglutination titres of immune and non immune sera.
If the experiment went perfectly, do u know what would results i'd get?Please dont think im cheating, I misplaced the results,I've only got a photo of the haemagglutination tray.

method:
The immune rabbit serum was heated for 30 mins, to inactivate natural complement present in the serum.Then we made a serial dilution of the rabbit serum in phosphate buffered saline (PBS). Then placed 0.2ml from each dilution into a well in the hemaglutination tray.To each well we added 0.2ml of a 2% solution of sheep red blood cells in PBS pH7.2. Then we set up control wells containing 0.2ml dilution buffer and 0.2ml cell suspension. Then repeated method with non immune serum but with fewer dilutions.

can anyone help me please?
For my results I have to read the agglutination titre in the serum. This may be taken at the lowest dilution at which agglutination is evident. I know the control wells will give a negative result. I need to determine the agglutinations titres of immune and non immune sera defining the end point.. Thanku for any help given, I really appreciate it..xx

Answer 1

When there is no antibody in the serum sample, the sheep blood cells settle at the center of the well, and you see a red dot. When antibodies are present, each immunoglobulin binds to two blood cells, and when there are enough antibodies, the resulting structure looks much like a fish net. When you look from above, you do not see anything. So a red dot is negative, and no dot means positive. The last dilution that gives a positive reaction is your titer.

The serum samples are heat-inactivated to destroy the complement to prevent the lysis of the sheep blood cells.