PLEASE HELP me science/"digestion" nerds!! Im in a big rut ='-(?

Question

To make this short we did a lab & I totally screwed up.

We were supposed to: imitate digesting a boiled egg (protein) with given substances. (Only directions). We had Pepsinogen, HCl, Bile, Pancreatic solution and water. We were to use 4 test tubes, and observe the qualitative process of digestion over a few days.

What we did: put the chopped boiled egg in HCl for 5 minutes. Put it in water for 5 min. Put it in Bile for 5 min and then put it in pancreatic solution for 2 days. Please dont laugh at me...but here;s my list of mistakes:

1. Didn't make the necessary Pepsin by mixing pepsinogen and HCl. In fact I didnt use Pepsinogen at all cuz I forgot)!

2. We didn't have a controlled factor (which should have been amount of HCL)...in which case pepsinogen would be independent.

3. Even if we did do that we needed to leave it in for longer than 5 min.

4. There is no pancreatic solution in protein digestion!!
HELP!
I have no control variable & can only discus 1 error!TY

Answer 1

The error is in the sequence:
Protein digestion in the digestive tract, is initiated in the stomach, with the presence of hydrochloric acid, with Ph adjusted to 2-3. and in the presence of pepsine (pepsinogen)
The temperature for this, should be near the normal body temperature during the whole process, (this step and the ones that follow)
The next step, also in body temperature environment, is to use amylase, pancreatine (available in tablets at the pharmacyst), and bile....however, because the bile is a natural detergent that dissolves fats (contains taurocolic and glycocolic acids) and carboxypeptidase....
For this next step, you need to make the environment slightly alkaline (Ph=8), and in the human or other body, this is achieved by the alkaline mucus secreted by the intestinal wall....
Adjust your Ph with a buffer such as sodium phosphate 1 Molar concentration.....keep it at body temperature, SHAKE it constantly, (there are lab machines that are designed for the purpose), so that enzymes can be in contact with the substrate (such as it happens in normal intestinal motion), otherwise, they will not react..

Answer 2

You are talking about normal digestion of denatured proteins (albumin)

The error is in the sequence:
Protein digestion in the digestive tract, is initiated in the stomach, with the presence of hydrochloric acid, with Ph adjusted to 2-3. and in the presence of pepsine (pepsinogen)
The temperature for this, should be near the normal body temperature during the whole process, (this step and the ones that follow)
The next step, also in body temperature environment, is to use amylase, pancreatine (available in tablets at the pharmacyst), and bile....however, because the bile is a natural detergent that dissolves fats (contains taurocolic and glycocolic acids) and carboxypeptidase....
For this next step, you need to make the environment slightly alkaline (Ph=8), and in the human or other body, this is achieved by the alkaline mucus secreted by the intestinal wall....
Adjust your Ph with a buffer such as sodium phosphate 1 Molar concentration.....keep it at body temperature, SHAKE it constantly, (there are lab machines that are designed for the purpose), so that enzymes can be in contact with the substrate (such as it happens in normal intestinal motion), otherwise, they will not react..

Answer 3

nerd??