The isoelectri point for normal hemoglobin is 6.9. The electrophoresis buffer for this experiment has a pH of 9.2.
a. What charge does the hemoglobin molecule have at pH 9.2? Explain
b. How would hemoglobin migrate if the electrophoresis buffer had a pH of 5.0?
c. How would normal hemoglobin migrate if the electrophoresis buffer had a pH of 6.9? Explain.
2007-10-16 02:02:32 · 2 answers · asked by asdf1234 2 in Science & Mathematics ➔ Chemistry
a. Minus, (-), overall. There might be several minus charges on the molecule.
b. The opposite from that at pH 9.2.
c. It would not migrate. That is the meaning of isoelectric point. All of the R-NH3+ groups from basic amino acids would balance out all the -COO(-) groups from acidic amino acids.
2007-10-16 02:56:29 · answer #1 · answered by steve_geo1 7 · 0⤊ 0⤋
Hemoglobin Isoelectric Point
2016-11-10 09:38:50 · answer #2 · answered by Anonymous · 0⤊ 0⤋