what is the third steps of cell fractionation?

Answer 1

1.Homogenization
2.Centrifugation
3.Collection of a set number of drops of the centrifuged stuff in different test tubes. This is done by puncturing the hole at the bottom of the centrifuged tube containing the cytosol.

Answer 2

Cell fractionation is the separation of homogeneous sets, usually organelles, from a heterogeneous population of cells.

Steps
1.Disruption (homogenisation) of cells and 2. liberation of organelles.
3.Macro Filtration
4.Purification of cell components.
the sequential increase in gravitational force resulting in the sequential separation of organelles according to their density.